OIVA (P01397) Protein Card

General Information
Name OIVA
Alternative name(s) O4a
Organism Conus obscurus
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Notes The superfamily assignment is not supported by any available data but the cDNA extraction method is based on the conservation of 5' and 3' UTR.

Classification
Conopeptide class conotoxin
Gene superfamily A superfamily
Cysteine framework IV
Pharmacological family alpha conotoxin

Sequence
CCGVONAACHOCVCKNTC(nh2)
Modified residues
positionsymbolname
5O4-Hydroxyproline
11O4-Hydroxyproline
19nh2C-term amidation
Sequence evidence protein level
Average Mass 1848.14
Monoisotopic Mass 1846.65
Isoelectric Point 10.95
Extinction Coefficient [280nm] NA

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α1β1δεM. musculus310nM+/-801-10uM AchTeichert,R.W. et al. (2006)
α1β1γδM. musculus510pM+/-1301-10uM AchTeichert,R.W. et al. (2006)

Percentage inhibition: 5-HT3 receptors

TargetOrganism% inhibitionConcentrationAgonistRef
5-HT3AH. sapiens90+/-1010uM3uM serotoninTeichert,R.W. et al. (2006)

Percentage inhibition: Nicotinic acetylcholine receptors

TargetOrganism% inhibitionConcentrationAgonistRef
α1β1δεH. sapiens10010uM1-10uM AchTeichert,R.W. et al. (2006)
M. musculus9010uM1-10uM AchTeichert,R.W. et al. (2006)
α1β1γδH. sapiens10010uM1-10uM AchTeichert,R.W. et al. (2006)
M. musculus10010uM1-10uM AchTeichert,R.W. et al. (2006)
α2β4H. sapiens010uM100uM AchTeichert,R.W. et al. (2006)
α3β4H. sapiens010uM100uM AchTeichert,R.W. et al. (2006)
α4β2H. sapiens5+/-510uM100uM AchTeichert,R.W. et al. (2006)
M. musculus10+/-1010uM100uM AchTeichert,R.W. et al. (2006)
α6/α3β2β3R. norvegicus010uM100uM AchTeichert,R.W. et al. (2006)
α7H. sapiens010uM200uM AchTeichert,R.W. et al. (2006)

Synthetic variants
OIVA [H10P](nh2)CTNKCVCOPCAANOVGCC
OIVA [K15N](nh2)CTNNCVCOHCAANOVGCC

References
Teichert,R.W., Rivier,J., Dykert,J., Cervini,L., Gulyas,J., Bulaj,G., Ellison,M. and Olivera,B.M. (2004) AlphaA-Conotoxin OIVA defines a new alphaA-conotoxin subfamily of nicotinic acetylcholine receptor inhibitors Toxicon 44:207-214
Teichert,R.W., Lopez-Vera,E., Gulyas,J., Watkins,M., Rivier,J. and Olivera,B.M. (2006) Definition and characterization of the short alphaA-conotoxins: a single residue determines dissociation kinetics from the fetal muscle nicotinic acetylcholine receptor Biochemistry 45:1304-1312

Internal links
Nucleic acids
Structure

External links
UniProtKB/Swiss-Prot P69746
Ncbi P69746

Tools