PIVA (P01449) Protein Card

General Information
Name PIVA
Alternative name(s) P4a
Organism Conus purpurascens
Organism region Eastern Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor PIVA precursor (4222)

Classification
Conopeptide class conotoxin
Gene superfamily A superfamily
Cysteine framework IV
Pharmacological family alpha conotoxin

Sequence
GCCGSYONAACHOCSCKDROSYCGQ(nh2)
Modified residues
positionsymbolname
7O4-Hydroxyproline
13O4-Hydroxyproline
20O4-Hydroxyproline
26nh2C-term amidation
Sequence evidence protein level
Average Mass 2648.88
Monoisotopic Mass 2646.92
Isoelectric Point 9.49
Extinction Coefficient [280nm] 2980.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α1β1δεM. musculus2.3nM+/-0.71-10uM AchTeichert,R.W. et al. (2006)
α1β1γδM. musculus22nM+/-41-10uM AchTeichert,R.W. et al. (2006)

References
Teichert,R.W., Lopez-Vera,E., Gulyas,J., Watkins,M., Rivier,J. and Olivera,B.M. (2006) Definition and characterization of the short alphaA-conotoxins: a single residue determines dissociation kinetics from the fetal muscle nicotinic acetylcholine receptor Biochemistry 45:1304-1312
Hopkins,C., Grilley,M., Miller,C., Shon,K.J., Cruz,L.J., Gray,W.R., Dykert,J., Rivier,J., Yoshikami,D. and Olivera,B.M. (1995) A new family of Conus peptides targeted to the nicotinic acetylcholine receptor J. Biol. Chem. 270:22361-22367
Han,K.H., Hwang,K.J., Kim,S.M., Kim,S.K., Gray,W.R., Olivera,B.M., Rivier,J. and Shon,K.J. (1997) NMR structure determination of a novel conotoxin, [Pro 7,13] alpha A-conotoxin PIVA Biochemistry 36:1669-1677

Internal links
Protein Precursor PIVA precursor (4222)
Nucleic acids
Structure

External links
UniProtKB/Swiss-Prot P55963
Ncbi P55963

Tools