RVIIIA (P01574) Protein Card

General Information
Name RVIIIA
Alternative name(s) R8a
Organism Conus radiatus
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor RVIIIA precursor (4000)
Notes RVIIIA has no activity against the glutamate-activated chloride channels.

Classification
Conopeptide class conotoxin
Gene superfamily S superfamily
Cysteine framework VIII
Pharmacological family alpha conotoxin

Sequence
KCNFDKCKGTGVYNCG(Gla)SCSC(Gla)GLHSCRCTYNI
GSMKSGCACICTYY
Modified residues
positionsymbolname
17GlaGamma carboxylic glutamic acid
22GlaGamma carboxylic glutamic acid
Sequence evidence protein level
Average Mass 5170.75
Monoisotopic Mass 5166.93
Isoelectric Point 9.46
Extinction Coefficient [280nm] 5960.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α1β1δεH. sapiens100nM1uM AchTeichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
α1β1γδH. sapiens33nM2uM AchTeichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)

Percentage inhibition: 5-HT3 receptors

TargetOrganism% inhibitionConcentrationAgonistRef
5-HT3AH. sapiens010uMTeichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)

Percentage inhibition: Nicotinic acetylcholine receptors

TargetOrganism% inhibitionConcentrationAgonistRef
α3β2H. sapiens6510uM100uM AchTeichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
R. norvegicus5010uM100uM AchTeichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
α3β4H. sapiens2010uM100uM AchTeichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
α4β2H. sapiens2010uM100uM AchTeichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
α6/α3β2β3R. norvegicus5010uM100uM AchTeichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
α7H. sapiens701uM100uM AchTeichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)
9010uM 100uM AchTeichert,R.W. and Jimenez,E.C. and Olivera,B.M. (2005)

References
Teichert,R.W., Jimenez,E.C. and Olivera,B.M. (2005) Alpha S-conotoxin RVIIIA: a structurally unique conotoxin that broadly targets nicotinic acetylcholine receptors Biochemistry 44:7897-7902

Internal links
Protein Precursor RVIIIA precursor (4000)
Nucleic acids
Structure

External links
UniProtKB/Swiss-Prot P0C1W3
Ncbi P0C1W3

Tools