PIIIF (P01594) Protein Card

General Information
Name PIIIF
Alternative name(s) P3f
Organism Conus purpurascens
Organism region Eastern Pacific
Organism diet piscivorous
Protein Type Wild type

Classification
Conopeptide class conotoxin
Gene superfamily M superfamily
Cysteine framework III
Pharmacological family alpha conotoxin

Sequence
GOOCCLYGSCROFOGCYNALCCRK(nh2)
Modified residues
positionsymbolname
2O4-Hydroxyproline
3O4-Hydroxyproline
12O4-Hydroxyproline
14O4-Hydroxyproline
25nh2C-term amidation
Sequence evidence protein level
Average Mass 2666.08
Monoisotopic Mass 2664.06
Isoelectric Point 10.72
Extinction Coefficient [280nm] 2980.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α1β1γδT. marmorata19.0uM+/-0.91uM AchVan Wagoner,R.M. et al. (2003)

Synthetic variants
PIIIF [O12R,F13Y](nh2)KRCCLANYCGOYRRCSGYLCCOOG
PIIIF [S9K](nh2)KRCCLANYCGOFORCSGYLCCOOG
PIIIF [Y17S,N18S,L20S](nh2)KRCCSASSCGOFORCSGYLCCOOG

References
Van Wagoner,R.M., Jacobsen,R.B., Olivera,B.M. and Ireland,C.M. (2003) Characterization and three-dimensional structure determination of psi-conotoxin Piiif, a novel noncompetitive antagonist of nicotinic acetylcholine receptors Biochemistry 42:6353-6362

Internal links
Nucleic acids
Structure SOLUTION STRUCTURE OF THE NONCOMPETITIVE SKELETAL MUSCLE NICOTINIC ACETYLCHOLINE RECEPTOR ANTAGONIST PSI-CONOTOXIN PIIIF

External links
UniProtKB/Swiss-Prot P60245
Ncbi P60245, 1JLP_A

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