PIIIE (P01611) Protein Card

General Information
Name PIIIE
Alternative name(s) P3.8,P3e
Organism Conus purpurascens
Organism region Eastern Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor PIIIE precursor (1406)

Classification
Conopeptide class conotoxin
Gene superfamily M superfamily
Cysteine framework III
Pharmacological family alpha conotoxin

Sequence
HOOCCLYGKCRRYOGCSSASCCQR(nh2)
Modified residues
positionsymbolname
2O4-Hydroxyproline
3O4-Hydroxyproline
14O4-Hydroxyproline
25nh2C-term amidation
Sequence evidence protein level
Average Mass 2717.09
Monoisotopic Mass 2715.08
Isoelectric Point 11.55
Extinction Coefficient [280nm] 2980.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α1β1δεM. musculus7.4uM100uM AchLluisma,A.O. et al. (2008)
α1β1γδM. musculus>10uM100uM AchLluisma,A.O. et al. (2008)
T. marmorata177nM+/-31uM AchVan Wagoner,R.M. et al. (2003)

Synthetic variants
PIIIE [K9S]HOOCCLYGKCRRYOGCSSASCCQR(nh2)
PIIIE [R12O,Y13F]HOOCCLYGKCROFOGCSSASCCQR(nh2)
PIIIE [S17Y,S18N,S20L]HOOCCLYGKCRRYOGCSSASCCQR(nh2)

References
Shon,K.J., Grilley,M., Jacobsen,R., Cartier,G.E., Hopkins,C., Gray,W.R., Watkins,M., Hillyard,D.R., Rivier,J., Torres,J., Yoshikami,D. and Olivera,B.M. (1997) A noncompetitive peptide inhibitor of the nicotinic acetylcholine receptor from Conus purpurascens venom Biochemistry 36:9581-9587
Mitchell,S.S., Shon,K.J., Foster,M.P., Davis,D.R., Olivera,B.M. and Ireland,C.M. (1998) Three-dimensional solution structure of conotoxin psi-PIIIE, an acetylcholine gated ion channel antagonist Biochemistry 37:1215-1220
Van Wagoner,R.M. and Ireland,C.M. (2003) An improved solution structure for psi-conotoxin PiiiE. Biochemistry 42:6347-6352

Internal links
Protein Precursor PIIIE precursor (1406)
Nucleic acids
Structure SOLUTION STRUCTURE OF CONOTOXIN Y-PIIIE FROM CONUS PURPURASCENS, NMR, 14 STRUCTURES
SOLUTION STRUCTURE OF THE NONCOMPETITIVE SKELETAL MUSCLE NICOTINIC ACETYLCHOLINE RECEPTOR ANTAGONIST PSI-CONOTOXIN PIIIE

External links
Ncbi 1AS5_A, 1JLO_A

Tools