MIIIA (P01691) Protein Card

General Information
Name MIIIA
Alternative name(s) M3a
Organism Conus magus (Magus cone)
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Notes Block tetrodotoxin resistant (TTX-R) Na channels and the blockade is slow and reversible (Zhang et al 2006).

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework III
Pharmacological family mu conotoxin

Sequence
ZGCCNVPNGCSGRWCRDHAQCC(nh2)
Modified residues
positionsymbolname
1ZPyroglutamic acid
23nh2C-term amidation
Sequence evidence protein level
Average Mass 2373.62
Monoisotopic Mass 2371.83
Isoelectric Point NA
Extinction Coefficient [280nm] NA

Activity

IC50: Voltage-Gated Sodium Channels

TargetOrganismIC50Ref
Nav1.1R. norvegicus22.6uM+/-3.4Wilson,M.J. et al. (2011)
Nav1.5R. norvegicus>10uMWilson,M.J. et al. (2011)
Nav1.6M. musculus21.6uM+/-3.1Wilson,M.J. et al. (2011)
Nav1.7R. norvegicus97.0uM+/-10.5Wilson,M.J. et al. (2011)
Nav1.8R. norvegicus>10uMWilson,M.J. et al. (2011)

Kd: Voltage-Gated Sodium Channels

TargetOrganismKdRef
Nav1.2R. norvegicus450nM+/-180Wilson,M.J. et al. (2011)
Nav1.3R. norvegicus7.7uM+/-0.8Wilson,M.J. et al. (2011)
Nav1.4R. norvegicus330nM+/-190Wilson,M.J. et al. (2011)

Percentage inhibition: Voltage-Gated Sodium Channels

TargetOrganism% inhibitionConcentrationRef
Nav TTX-RR. pipiens64+/-125uMZhang,M.M. et al. (2006)

References
Zhang,M.M., Fiedler,B., Green,B.R., Catlin,P., Watkins,M., Garrett,J.E., Smith,B.J., Yoshikami,D., Olivera,B.M. and Bulaj,G. (2006) Structural and functional diversities among mu-conotoxins targeting TTX-resistant sodium channels Biochemistry 45:3723-3732
Wilson,M.J., Yoshikami,D., Azam,L., Gajewiak,J., Olivera,B.M., Bulaj,G. and Zhang,M.M. (2011) μ-Conotoxins that differentially block sodium channels NaV1.1 through 1.8 identify those responsible for action potentials in sciatic nerve. Proc. Natl. Acad. Sci. U.S.A. 108:10302-10307

Internal links
Nucleic acids
Structure

External links
UniProtKB/Swiss-Prot P0C1U2
Ncbi P0C1U2

Tools