GIIIC (P01744) Protein Card

General Information
Name GIIIC
Alternative name(s) G3c
Organism Conus geographus (Geography cone)
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Notes Bind to tetrodotoxin sensitive Na channel (Cruz et al 1985) and, presumably, interact with site 1 in the Na channel pore.

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework III
Pharmacological family mu conotoxin

Sequence
RDCCTOOKKCKDRRCKOLKCCA(nh2)
Modified residues
positionsymbolname
6O4-Hydroxyproline
7O4-Hydroxyproline
17O4-Hydroxyproline
23nh2C-term amidation
Sequence evidence protein level
Average Mass 2594.11
Monoisotopic Mass 2592.18
Isoelectric Point 11.94
Extinction Coefficient [280nm] NA

Activity

IC50: Voltage-Gated Sodium Channels

TargetOrganismIC50nhillRef
Nav1.4R. norvegicus286 nM+/-131.21Harvey,P.J. et al. (2018)

Percentage inhibition: Voltage-Gated Sodium Channels

TargetOrganism% inhibitionConcentrationRef
Nav1.2R. norvegicus01 uMHarvey,P.J. et al. (2018)
Nav1.4R. norvegicus18+/-21 uMHarvey,P.J. et al. (2018)
Nav1.5R. norvegicus01 uMHarvey,P.J. et al. (2018)
Nav1.6R. norvegicus141 uMHarvey,P.J. et al. (2018)
Nav1.7R. norvegicus01 uMHarvey,P.J. et al. (2018)
Nav1.8R. norvegicus01 uMHarvey,P.J. et al. (2018)

References
Cruz,L.J., Gray,W.R., Olivera,B.M., Zeikus,R.D., Kerr,L., Yoshikami,D. and Moczydlowski,E. (1985) Conus geographus toxins that discriminate between neuronal and muscle sodium channels J. Biol. Chem. 260:9280-9288
Harvey,P.J., Kurniawan,N.D., Finol-Urdaneta,R.K., McArthur,J.R., Van Lysebetten,D., Dash,T.S., Hill,J.M., Adams,D.J., Durek,T. and Craik,D.J. (2018) NMR Structure of μ-Conotoxin GIIIC: Leucine 18 Induces Local Repacking of the N-Terminus Resulting in Reduced NaV Channel Potency Molecules 23:2715

Internal links
Nucleic acids
Structure NMR Solution structure of GIIIC

External links
UniProtKB/Swiss-Prot P05482
Ncbi P05482

Tools