CVID (P02549) Protein Card

General Information
Name CVID
Alternative name(s) AM-336,AM336,leconotide
Organism Conus catus
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor CVID precursor (1569)
CVID precursor (2872)

Classification
Conopeptide class conotoxin
Gene superfamily O1 superfamily
Cysteine framework VI/VII
Pharmacological family omega conotoxin

Sequence
CKSKGAKCSKLMYDCCSGSCSGTVGRC(nh2)
Modified residues
positionsymbolname
28nh2C-term amidation
Sequence evidence protein level
Average Mass 2756.22
Monoisotopic Mass 2754.13
Isoelectric Point 11.22
Extinction Coefficient [280nm] 1490.00

Activity

IC50: Voltage-Gated Calcium Channels

TargetOrganismIC50AgonistRef
Cav2.1R. norvegicus55uMMVIICLewis,R.J. et al. (2000)
Cav2.2R. norvegicus70pMGVIALewis,R.J. et al. (2000)
38pMGVIAMould,J. et al. (2004)

Synthetic variants
CVID[K10R]CKSKGAKCSRLMYDCCSGSCSGTVGRC(nh2)
CVID[K2A,K10R]CASKGAKCSRLMYDCCSGSCSGTVGRC(nh2)

References
Lewis,R.J., Nielsen,K.J., Craik,D.J., Loughnan,M.L., Adams,D.A., Sharpe,I.A., Luchian,T., Adams,D.J., Bond,T., Thomas,L., Jones,A., Matheson,J.L., Drinkwater,R., Andrews,P.R. and Alewood,P.F. (2000) Novel omega-conotoxins from Conus catus discriminate among neuronal calcium channel subtypes J. Biol. Chem. 275:35335-35344
Adams,D.J., Smith,A.B., Schroeder,C.I., Yasuda,T. and Lewis,R.J. (2003) Omega-conotoxin CVID inhibits a pharmacologically distinct voltage-sensitive calcium channel associated with transmitter release from preganglionic nerve terminals J. Biol. Chem. 278:4057-4062
Mould,J., Yasuda,T., Schroeder,C.I., Beedle,A.M., Doering,C.J., Zamponi,G.W., Adams,D.J. and Lewis,R.J. (2004) The alpha2delta auxiliary subunit reduces affinity of omega-conotoxins for recombinant N-type (Cav2.2) calcium channels. J. Biol. Chem. 279:34705-34714

Internal links
Protein Precursor CVID precursor (1569)
CVID precursor (2872)
Nucleic acids
Structure NMR structure of CVID

External links
Ncbi P58920

Tools