CVID (P02549) Protein Card

General Information
Name CVID
Alternative name(s) AM-336,AM336,Ca061,leconotide
Organism Conus catus
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor CVID precursor (1569)
CVID precursor (2872)

Classification
Conopeptide class conotoxin
Gene superfamily O1 superfamily
Cysteine framework VI/VII
Pharmacological family omega conotoxin

Sequence
CKSKGAKCSKLMYDCCSGSCSGTVGRC(nh2)
Modified residues
positionsymbolname
28nh2C-term amidation
Sequence evidence protein level
Average Mass 2756.22
Monoisotopic Mass 2754.13
Isoelectric Point 11.59
Extinction Coefficient [280nm] 1490.00

Activity

IC50: Voltage-Gated Calcium Channels

TargetOrganismIC50AgonistRef
Cav2.1R. norvegicus55uMMVIICLewis,R.J. et al. (2000)
Cav2.2R. norvegicus70pMGVIALewis,R.J. et al. (2000)
38pMGVIAMould,J. et al. (2004)

Synthetic variants
CVID[K10R]CKSKGAKCSRLMYDCCSGSCSGTVGRC(nh2)
CVID[K2A,K10R]CASKGAKCSRLMYDCCSGSCSGTVGRC(nh2)

References
Lewis,R.J., Nielsen,K.J., Craik,D.J., Loughnan,M.L., Adams,D.A., Sharpe,I.A., Luchian,T., Adams,D.J., Bond,T., Thomas,L., Jones,A., Matheson,J.L., Drinkwater,R., Andrews,P.R. and Alewood,P.F. (2000) Novel omega-conotoxins from Conus catus discriminate among neuronal calcium channel subtypes J. Biol. Chem. 275:35335-35344
Adams,D.J., Smith,A.B., Schroeder,C.I., Yasuda,T. and Lewis,R.J. (2003) Omega-conotoxin CVID inhibits a pharmacologically distinct voltage-sensitive calcium channel associated with transmitter release from preganglionic nerve terminals J. Biol. Chem. 278:4057-4062
Mould,J., Yasuda,T., Schroeder,C.I., Beedle,A.M., Doering,C.J., Zamponi,G.W., Adams,D.J. and Lewis,R.J. (2004) The alpha2delta auxiliary subunit reduces affinity of omega-conotoxins for recombinant N-type (Cav2.2) calcium channels. J. Biol. Chem. 279:34705-34714
Himaya, S.W.A., Jin, A.H., Dutertre, S., Giacomotto, J., Mohialdeen, H., Vetter, I., Alewood, P.F. and Lewis, R.J. (2015) Comparative venomics reveals the complex prey capture strategy of the piscivorous cone snail Conus catus. Journal of proteome research 14:4372-4381

Internal links
Protein Precursor CVID precursor (1569)
CVID precursor (2872)
Nucleic acids
Structure NMR structure of CVID

External links
Ncbi P58920

Tools