TxIXA (P02559) Protein Card

General Information
Name TxIXA
Alternative name(s) Tx9.1,Tx9a
Organism Conus textile (Cloth-of-gold cone)
Organism region Indo-Pacific
Organism diet molluscivorous
Protein Type Wild type
Protein precursor TxIXA precursor (608)
Notes The GmIXA peptide differs by the absence of the two gamma carboxylic acids. GmIXA does not present a difference of activity but TxIXA folds more efficiently than GmIXA in the presence of calcium ions (as in the endoplasmic reticulum).

Ueberheide et al. 2009 have isolated TxIXA without the modification of glutamic acids into γ-carboxylic glutamic acids.


Classification
Conopeptide class conotoxin
Gene superfamily P superfamily
Cysteine framework IX
Pharmacological family

Sequence
GCNNSCQ(Gla)HSDC(Gla)SHCICTFRGCGAVN(nh2)
Modified residues
positionsymbolname
8GlaGamma carboxylic glutamic acid
13GlaGamma carboxylic glutamic acid
28nh2C-term amidation
Sequence evidence protein level
Average Mass 2956.09
Monoisotopic Mass 2954.00
Isoelectric Point 5.47
Extinction Coefficient [280nm] NA

References
Miles,L.A., Dy,C.Y., Nielsen,J., Barnham,K.J., Hinds,M.G., Olivera,B.M., Bulaj,G. and Norton,R.S. (2002) Structure of a Novel P-superfamily Spasmodic Conotoxin Reveals an Inhibitory Cystine Knot Motif J. Biol. Chem. 277:43033-43040
Lirazan,M.B., Hooper,D., Corpuz,G.P., Ramilo,C.A., Bandyopadhyay,P., Cruz,L.J. and Olivera,B.M. (2000) The spasmodic peptide defines a new conotoxin superfamily Biochemistry 39:1583-1588
Ueberheide,B.M., Fenyƶ,D., Alewood,P.F. and Chait,B.T. (2009) Rapid sensitive analysis of cysteine rich peptide venom components. Proc. Natl. Acad. Sci. U.S.A. 106:6910-6915
Dobson,R., Collodoro,M., Gilles,N., Turtoi,A., De Pauw,E. and Quinton,L. (2012) Secretion and maturation of conotoxins in the venom ducts of Conus textile. Toxicon 60:1370-1379

Internal links
Protein Precursor TxIXA precursor (608)
Nucleic acids Conus textile spasmodic peptide tx9a mRNA, complete cds
Structure

External links
Ncbi Q9GU58, AAG28406

Tools