ImII (P02586) Protein Card

General Information
Name ImII
Organism Conus imperialis
Organism region Indo-Pacific
Organism diet vermivorous
Protein Type Wild type
Protein precursor ImII precursor (92)

Classification
Conopeptide class conotoxin
Gene superfamily A superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
ACCSDRRCRWRC(nh2)
Modified residues
positionsymbolname
13nh2C-term amidation
Sequence evidence nucleic acid level
Average Mass 1509.76
Monoisotopic Mass 1508.61
Isoelectric Point 12.50
Extinction Coefficient [280nm] 5500.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α1β1δεH. sapiens1.06uM1.0250nM AchEllison,M. et al. (2004)
9.5uM+/-3.8AchKasheverov,I.E. et al. (2006)
α2β2H. sapiens>10uM100uM AchEllison,M. et al. (2004)
α2β4H. sapiens>10uM100uM AchEllison,M. et al. (2004)
α3β2H. sapiens9.61uM1.1100uM AchEllison,M. et al. (2004)
α3β4H. sapiens>10uM100uM AchEllison,M. et al. (2004)
α4β2H. sapiens>10uM100uM AchEllison,M. et al. (2004)
α4β4H. sapiens>10uM100uM AchEllison,M. et al. (2004)
α7H. sapiens571nM1.11200uM AchEllison,M. et al. (2004)
1.5uMAchKasheverov,I.E. et al. (2009)
R. norvegicus441 nM1.195200 uM AchEllison,M.A. and McIntosh,J.M. and Olivera,B.M. (2003)

Synthetic variants
ImII [W10Y]ACCSDRRCRYRC(nh2)
ImII-isoACCSDRRCRWRC(nh2)

References
Kasheverov,I.E., Chiara,D.C., Zhmak,M.N., Maslennikov,I.V., Pashkov,V.S., Arseniev,A.S., Utkin,Y.N., Cohen,J.B. and Tsetlin,V.I. (2006) alpha-Conotoxin GI benzoylphenylalanine derivatives. (1)H-NMR structures and photoaffinity labeling of the Torpedo californica nicotinic acetylcholine receptor FEBS J. 273:1373-1388
Ellison,M.A., McIntosh,J.M. and Olivera,B.M. (2003) alpha-Conotoxins ImI and ImII: similar alpha 7 nicotinic receptor antagonists act at different sites J. Biol. Chem. 278:757-764
Ellison,M., Gao,F., Wang,H.L., Sine,S.M. and Olivera,B.M. (2004) Alpha-conotoxins ImI and ImII target distinct regions of the human alpha7 nicotinic acetylcholine receptor and distinguish human nicotinic receptor subtypes. Biochemistry 43:16019-16026

Internal links
Protein Precursor ImII precursor (92)
Nucleic acids
Structure

External links
Ncbi Q8I6R5, AAN78127

Tools