RIIIK (P02591) Protein Card

General Information
Name RIIIK
Alternative name(s) R3.1,R3k
Organism Conus radiatus
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor RIIIK precursor (1521)

Classification
Conopeptide class conotoxin
Gene superfamily M superfamily
Cysteine framework III
Pharmacological family kappa conotoxin

Sequence
LOSCCSLNLRLCOVOACKRNOCCT(nh2)
Modified residues
positionsymbolname
2O4-Hydroxyproline
13O4-Hydroxyproline
15O4-Hydroxyproline
21O4-Hydroxyproline
25nh2C-term amidation
Sequence evidence nucleic acid level
Average Mass 2649.14
Monoisotopic Mass 2647.16
Isoelectric Point 12.54
Extinction Coefficient [280nm] NA

Activity

IC50: Voltage-Gated Potassium Channels

TargetOrganismIC50Ref
Kv1.2O. mykiss73nM+/-34Al-Sabi,A. et al. (2004)

Synthetic variants
Ac-L1(nh2)TCCONRKCAOVOCLRLNLSCCSOL(Ac)
RIIIK [K18A](nh2)TCCONRACAOVOCLRLNLSCCSOL
RIIIK [K18R, R19K](nh2)TCCONKRCAOVOCLRLNLSCCSOL
RIIIK [L11A](nh2)TCCONRKCAOVOCLRLNLSCCSOL
RIIIK [L1A](nh2)TCCONRKCAOVOCLRLNLSCCSOA
RIIIK [L1E](nh2)TCCONRKCAOVOCLRLNLSCCSOE
RIIIK [L1F](nh2)TCCONRKCAOVOCLRLNLSCCSOF
RIIIK [L1H](nh2)TCCONRKCAOVOCLRLNLSCCSOH
RIIIK [L1I](nh2)TCCONRKCAOVOCLRLNLSCCSOI
RIIIK [L1K](nh2)TCCONRKCAOVOCLRLNLSCCSOK
RIIIK [L1M](nh2)TCCONRKCAOVOCLRLNLSCCSOM
RIIIK [L1R](nh2)TCCONRKCAOVOCLRLNLSCCSOR
RIIIK [L1Y](nh2)TCCONRKCAOVOCLRLNLSCCSOY
RIIIK [L7A](nh2)TCCONRKCAOVOCLRLNASCCSOL
RIIIK [L9A](nh2)TCCONRKCAOVOCLRANLSCCSOL
RIIIK [N20A](nh2)TCCOARKCAOVOCLRLNLSCCSOL
RIIIK [N8A](nh2)TCCONRKCAOVOCLRLALSCCSOL
RIIIK [O13A](nh2)TCCONRKCAOVACLRLNLSCCSOL
RIIIK [O15A](nh2)TCCONRKCAOVOCLRLNLSCCSOL
RIIIK [O21A](nh2)TCCANRKCAOVOCLRLNLSCCSOL
RIIIK [O2A](nh2)TCCONRKCAOVOCLRLNLSCCSAL
RIIIK [R10A](nh2)TCCONRKCAOVOCLALNLSCCSOL
RIIIK [R19A](nh2)TCCONAKCAOVOCLRLNLSCCSOL
RIIIK [S3A](nh2)TCCONRKCAOVOCLRLNLSCCAOL
RIIIK [S6A](nh2)TCCONRKCAOVOCLRLNLACCSOL
RIIIK [T24A](nh2)ACCONRKCAOVOCLRLNLSCCSOL
RIIIK [V14A](nh2)TCCONRKCAOAOCLRLNLSCCSOL
RIIIKΔ9(nh2)TCCONRKCAOVOCLRLNLSCCSOL

References
Corpuz,G.P., Jacobsen,R.B., Jimenez,E.C., Watkins,M., Walker,C., Colledge,C., Garrett,J.E., McDougal,O., Li,W., Gray,W.R., Hillyard,D.R., Rivier,J., McIntosh,J.M., Cruz,L.J. and Olivera,B.M. (2005) Definition of the M-conotoxin superfamily: characterization of novel peptides from molluscivorous Conus venoms Biochemistry 44:8176-8186
Ferber,M., Sporning,A., Jeserich,G., DeLaCruz,R., Watkins,M., Olivera,B.M. and Terlau,H. (2003) A novel conus peptide ligand for K+ channels J. Biol. Chem. 278:2177-2183
Al-Sabi,A., Lennartz,D., Ferber,M., Gulyas,J., Rivier,J.E., Olivera,B.M., Carlomagno,T. and Terlau,H. (2004) KappaM-conotoxin RIIIK, structural and functional novelty in a K+ channel antagonist. Biochemistry 43:8625-8635

Internal links
Protein Precursor RIIIK precursor (1521)
Nucleic acids
Structure

External links
Ncbi P69769

Tools