BuIA (P00026) Protein Card

General Information
Name BuIA
Alternative name(s) Bu1a
Organism Conus bullatus
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor BuIA precursor (409)

Classification
Conopeptide class conotoxin
Gene superfamily A superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
GCCSTPPCAVLYC(nh2)
Modified residues
positionsymbolname
14nh2C-term amidation
Sequence evidence nucleic acid level
Average Mass 1311.56
Monoisotopic Mass 1310.49
Isoelectric Point 8.09
Extinction Coefficient [280nm] 1490.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α2β2R. norvegicus800nM0.85100uM AchAzam,L. et al. (2005)
α2β4R. norvegicus121nM1.31100uM AchAzam,L. et al. (2005)
α3β2R. norvegicus5.72nM1.48100uM AchAzam,L. et al. (2005)
α3β4R. norvegicus27.7nM1.52100uM AchAzam,L. et al. (2005)
α4β2R. norvegicus10.4uM0.525100uM AchAzam,L. et al. (2005)
α4β4R. norvegicus69.9nM1.15100uM AchAzam,L. et al. (2005)
α6/α3β2R. norvegicus258pM0.963100uM AchAzam,L. et al. (2005)
α6/α3β2β3R. norvegicus460pM+/-1501.3100uM AchAzam,L. et al. (2010)
α6/α3β4R. norvegicus1.54nM1.4100uM AchAzam,L. et al. (2005)
2.1nM+/-0.41.7100uM AchAzam,L. et al. (2010)
α7R. norvegicus272nM1.21100uM AchAzam,L. et al. (2005)

Kd: Nicotinic acetylcholine receptors

TargetOrganismKdRef
α2β4R. norvegicus62.9nMAzam,L. et al. (2005)
α3β2R. norvegicus5.85nMShiembob,D.L. et al. (2006)
α3β4R. norvegicus23.8nMAzam,L. et al. (2005)
α4β4R. norvegicus21.8nMAzam,L. et al. (2005)
α6/α3β4R. norvegicus2.58nMAzam,L. et al. (2005)

Synthetic variants
BuIA[A9S]GCCSTPPCSVLYC(nh2)
BuIA[L11A]GCCSTPPCAVAYC(nh2)
BuIA[P6O]GCCSTOPCAVLYC(nh2)
BuIA[P7O]GCCSTPOCAVLYC(nh2)
BuIA[S4A]GCCATPPCAVLYC(nh2)
BuIA[T5A]GCCSAPPCAVLYC(nh2)
BuIA[V10A]GCCSTPPCAALYC(nh2)
BuIA[Y12A]GCCSTPPCAVLAC(nh2)

References
Chi,S.W., Kim,D.H., Olivera,B.M., McIntosh,J.M. and Han,K.H. (2006) NMR structure determination of alpha-conotoxin BuIA, a novel neuronal nicotinic acetylcholine receptor antagonist with an unusual 4/4 disulfide scaffold Biochem. Biophys. Res. Commun. 349:1228-1234
Azam,L., Dowell,C., Watkins,M., Stitzel,J.A., Olivera,B.M. and McIntosh,J.M. (2005) Alpha-conotoxin BuIA, a novel peptide from Conus bullatus, distinguishes among neuronal nicotinic acetylcholine receptors J. Biol. Chem. 280:80-87
Jin,A.H., Brandstaetter,H., Nevin,S.T., Tan,C.C., Clark,R.J., Adams,D.J., Alewood,P.F., Craik,D.J. and Daly,N.L. (2007) Structure of alpha-conotoxin BuIA: influences of disulfide connectivity on structural dynamics. BMC Struct. Biol. 7:28
Shiembob,D.L., Roberts,R.L., Luetje,C.W. and McIntosh,J.M. (2006) Determinants of alpha-conotoxin BuIA selectivity on the nicotinic acetylcholine receptor beta subunit. Biochemistry 45:11200-11207

Internal links
Protein Precursor BuIA precursor (409)
Nucleic acids
Structure SOLUTION STRUCTURE OF ALPHA-CONOTOXIN BUIA
SOLUTION STRUCTURE OF RIBBON BUIA
Crystal structure of acetylcholine binding protein (AChBP) from Aplysia Californica in complex with alpha-conotoxin BuIA

External links
Ncbi 4EZ1_O, 4EZ1_N, 4EZ1_L, 4EZ1_K, 2I28_A

Tools