LtIA (P02665) Protein Card

General Information
Name LtIA
Alternative name(s) Lt1.1,Lt1A
Organism Conus litteratus
Organism region Indo-Pacific
Organism diet vermivorous
Protein Type Wild type
Protein precursor LtIA precursor (1151)
Notes Lp1.1 from Conus leopardus has the same sequence as LtIA. The activity of Lp1.1 for different nAChR subtypes was also characterized.

Classification
Conopeptide class conotoxin
Gene superfamily A superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
GCCARAACAGIHQELC(nh2)
Modified residues
positionsymbolname
17nh2C-term amidation
Sequence evidence nucleic acid level
Average Mass 1600.86
Monoisotopic Mass 1599.65
Isoelectric Point 8.31
Extinction Coefficient [280nm] NA

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α1β1δεM. musculus>10uM10uM AchLuo,S. et al. (2010)
α2β2R. norvegicus>10uM100uM AchLuo,S. et al. (2010)
α2β4R. norvegicus>10uM100uM AchLuo,S. et al. (2010)
α3β2R. norvegicus9.79nM0.63100uM AchLuo,S. et al. (2010)
α3β4R. norvegicus>10uM100uM AchLuo,S. et al. (2010)
α4β2R. norvegicus>10uM100uM AchLuo,S. et al. (2010)
α4β4R. norvegicus>10uM100uM AchLuo,S. et al. (2010)
α6/α3β2β3R. norvegicus84.4nM0.76100uM AchLuo,S. et al. (2010)
α6/α3β4R. norvegicus6.0uM0.95100uM AchLuo,S. et al. (2010)
α7R. norvegicus>10uM200uM AchLuo,S. et al. (2010)
α9α10R. norvegicus>10uM10uM AchLuo,S. et al. (2010)

Synthetic variants
LtIA [A4S,A6P](nh2)CLEQHIGACAPRSCCG
LtIA [A4S](nh2)CLEQHIGACAARSCCG

References
Pi,C., Liu,J., Peng,C., Liu,Y., Jiang,X., Zhao,Y., Tang,S., Wang,L., Dong,M., Chen,S. and Xu,A. (2006) Diversity and evolution of conotoxins based on gene expression profiling of Conus litteratus Genomics 88:809-819
Luo,S., Akondi,K.B., Zhangsun,D., Wu,Y., Zhu,X., Hu,Y., Christensen,S., Dowell,C., Daly,N.L., Craik,D.J., Wang,C.I., Lewis,R.J., Alewood,P.F. and Michael McIntosh,J. (2010) Atypical alpha-conotoxin LtIA from Conus litteratus targets a novel microsite of the alpha3beta2 nicotinic receptor. J. Biol. Chem. 285:12355-12366

Internal links
Protein Precursor LtIA precursor (1151)
Nucleic acids
Structure

External links
Ncbi ABC74972

Tools