Reg1e (P00030) Protein Card

General Information
Name Reg1e
Alternative name(s) RgIA[Del13]-Amidated
Organism Conus regius
Organism region Eastern Pacific
Organism diet vermivorous
Protein Type Wild type
Notes RgIA precursor seems to be the precursor sequence of Reg1e. RgIA has been predicted from the protein prosequence (Ellison et al. 2006). RgIA presents an N-terminal arginine that is usually cleaved by an enzyme (CPE). Reg1e is therefore presumably the real mature peptide resulting from the natural processing of the "RgIA precursor". Inhibited HVA Ca2+ channel current via GABAB receptors in rat DRG neurons with an IC50 of 20.5 nM. (Halai 2011)

Classification
Conopeptide class conotoxin
Gene superfamily A superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
GCCSDORCRYRC(nh2)
Modified residues
positionsymbolname
6O4-Hydroxyproline
13nh2C-term amidation
Sequence evidence protein level
Average Mass 1429.62
Monoisotopic Mass 1428.53
Isoelectric Point 12.20
Extinction Coefficient [280nm] 1490.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α7R. norvegicus1.1uM0.9200uM AchEllison,M. et al. (2008)
α9α10R. norvegicus7.77nM1.350uM AchEllison,M. et al. (2008)

Percentage inhibition: Nicotinic acetylcholine receptors

TargetOrganism% inhibitionConcentrationRef
α9α10unidentified(nAChR was hybrid human α9/rat α10. )41+/-1550 nMHalai,R. et al. (2011)

References
Franco,A., Pisarewicz,K., Moller,C., Mora,D., Fields,G.B. and Mari,F. (2006) Hyperhydroxylation: a new strategy for neuronal targeting by venomous marine molluscs Prog. Mol. Subcell. Biol. 43:83
Ellison,M., Feng,Z.P., Park,A.J., Zhang,X., Olivera,B.M., McIntosh,J.M. and Norton,R.S. (2008) Alpha-RgIA, a novel conotoxin that blocks the alpha9alpha10 nAChR: structure and identification of key receptor-binding residues. J. Mol. Biol. 377:1216-1227
Halai,R., Callaghan,B., Daly,N.L., Clark,R.J., Adams,D.J. and Craik,D.J. (2011) Effects of cyclization on stability, structure, and activity of α-conotoxin RgIA at the α9α10 nicotinic acetylcholine receptor and GABA(B) receptor. J. Med. Chem. 54:6984-6992

Internal links
Nucleic acids
Structure

External links
UniProtKB/Swiss-Prot P85011
Ncbi P85011

Tools