SxIIIA (P03584) Protein Card

General Information
Name SxIIIA
Alternative name(s) StIIIA,Sx3A
Organism Conus striolatus
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Notes The superfamily assignment is not supported by any available data but the cDNA extraction method is based on the conservation of the signal sequence. 10 μM rapidly blocks A-fiber compound action potentials in mouse sciatic nerve, but not C-fiber CAPs [Wilson,M.J., Zhang,M. (2015)].

Classification
Conopeptide class conotoxin
Gene superfamily M superfamily
Cysteine framework III
Pharmacological family mu conotoxin

Sequence
RCCTGKKGSCSGRACKNLKCCA(nh2)
Modified residues
positionsymbolname
23nh2C-term amidation
Sequence evidence nucleic acid level
Average Mass 2270.72
Monoisotopic Mass 2268.99
Isoelectric Point 12.61
Extinction Coefficient [280nm] NA

Activity

IC50: Voltage-Gated Sodium Channels

TargetOrganismIC50Ref
Nav1.1R. norvegicus370nM+/-40Wilson,M.J. et al. (2011)
Nav1.2R. norvegicus1.0uM+/-0.1Wilson,M.J. et al. (2011)
Nav1.3R. norvegicus>10uMWilson,M.J. et al. (2011)
Nav1.4R. norvegicus7nM+/-1Walewska A et al. (2008)
Nav1.5R. norvegicus>10uMWilson,M.J. et al. (2011)
Nav1.6M. musculus(With rat β1 subunit.)0.48 μM+/-0.06Wilson,M.J. et al. (2015)
570nM+/-80Wilson,M.J. et al. (2011)
(With rat β3 subunit.)0.44 μM+/-0.01Wilson,M.J. et al. (2015)
(With rat β2 subunit.)7.74 μM+/-0.12Wilson,M.J. et al. (2015)
(With rat β1 + β4 subunit.)1.05 μM+/-0.05Wilson,M.J. et al. (2015)
(With rat β3 + β4 subunit.)1.08 μM+/-0.02Wilson,M.J. et al. (2015)
(With rat β3 + β2 subunit.)1.15 μM+/-0.01Wilson,M.J. et al. (2015)
(With rat β1 + β2 subunit.)2.32 μM+/-0.03Wilson,M.J. et al. (2015)
(With rat β4 subunit.)11.21 μM+/-0.3Wilson,M.J. et al. (2015)
R. norvegicus(With rat β1 subunit. )19.89 μM+/-3.24Wilson,M.J. et al. (2015)
Nav1.7R. norvegicus>10uMWilson,M.J. et al. (2011)
Nav1.8R. norvegicus>10uMWilson,M.J. et al. (2011)

References
Walewska A, Skalicky JJ, Davis DR, Zhang MM, Lopez-Vera E, Watkins M, Han TS, Yoshikami D, Olivera BM, Bulaj G (2008) NMR-based mapping of disulfide bridges in cysteine-rich peptides: application to the mu-conotoxin SxIIIA J Am Chem Soc 130:14280-14286
Wilson,M.J., Yoshikami,D., Azam,L., Gajewiak,J., Olivera,B.M., Bulaj,G. and Zhang,M.M. (2011) μ-Conotoxins that differentially block sodium channels NaV1.1 through 1.8 identify those responsible for action potentials in sciatic nerve. Proc. Natl. Acad. Sci. U.S.A. 108:10302-10307
Wilson,M.J., Zhang,M.M., Gajewiak,J., Azam,L., Rivier,J.E., Olivera,B.M. and Yoshikami,D. (2015) Α- and β-subunit composition of voltage-gated sodium channels investigated with μ-conotoxins and the recently discovered μO§-conotoxin GVIIJ. J. Neurophysiol. 113:2289-2301

Internal links
Nucleic acids
Structure

External links
UniProtKB/Swiss-Prot P0C8V2
Ncbi P0C8V2

Tools