BuIIIA (P03623) Protein Card

General Information
Name BuIIIA
Alternative name(s) Bu3A
Organism Conus bullatus
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor BuIIIA precursor (3716)

Classification
Conopeptide class conotoxin
Gene superfamily M superfamily
Cysteine framework III
Pharmacological family mu conotoxin

Sequence
VTDRCCKGKRECGRWCRDHSRCC(nh2)
Modified residues
positionsymbolname
24nh2C-term amidation
Sequence evidence nucleic acid level
Average Mass 2751.16
Monoisotopic Mass 2749.15
Isoelectric Point 12.01
Extinction Coefficient [280nm] 5500.00

Activity

IC50: Voltage-Gated Sodium Channels

TargetOrganismIC50Ref
Nav1.5R. norvegicus13.8uM+/-1.1Wilson,M.J. et al. (2011)
Nav1.6M. musculus4.4uM+/-0.5Wilson,M.J. et al. (2011)
Nav1.7R. norvegicus>10uMWilson,M.J. et al. (2011)
Nav1.8R. norvegicus>10uMWilson,M.J. et al. (2011)

Kd: Voltage-Gated Sodium Channels

TargetOrganismKdRef
Nav1.1R. norvegicus350nM+/-104Wilson,M.J. et al. (2011)
Nav1.2R. norvegicus12nM+/-4Wilson,M.J. et al. (2011)
Nav1.3R. norvegicus350nM+/-60Wilson,M.J. et al. (2011)
Nav1.4R. norvegicus12nM+/-3Wilson,M.J. et al. (2011)

References
Holford,M., Zhang,M.M., Gowd,K.H., Azam,L., Green,B.R., Watkins,M., Ownby,J.P., Yoshikami,D., Bulaj,G. and Olivera,B.M. (2009) Pruning nature: Biodiversity-derived discovery of novel sodium channel blocking conotoxins from Conus bullatus. Toxicon 53:90-98
Wilson,M.J., Yoshikami,D., Azam,L., Gajewiak,J., Olivera,B.M., Bulaj,G. and Zhang,M.M. (2011) μ-Conotoxins that differentially block sodium channels NaV1.1 through 1.8 identify those responsible for action potentials in sciatic nerve. Proc. Natl. Acad. Sci. U.S.A. 108:10302-10307

Internal links
Protein Precursor BuIIIA precursor (3716)
Nucleic acids
Structure

External links

Tools