CnIIIC (P03834) Protein Card

General Information
Name CnIIIC
Organism Conus consors
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor CnIIIC precursor (6155)

Classification
Conopeptide class conotoxin
Gene superfamily M superfamily
Cysteine framework III
Pharmacological family

Sequence
ZGCCNGPKGCSSKWCRDHARCC(nh2)
Modified residues
positionsymbolname
1ZPyroglutamic acid
23nh2C-term amidation
Sequence evidence protein level
Average Mass 2375.68
Monoisotopic Mass 2373.88
Isoelectric Point NA
Extinction Coefficient [280nm] NA

Activity

IC50: Voltage-Gated Sodium Channels

TargetOrganismIC50Ref
Nav1.4H. sapiens4.95nM+/-0.5Markgraf,R. et al. (2012)
R. norvegicus1.3nM+/-0.4Favreau P et al. (2012)

Percentage inhibition: Voltage-Gated Sodium Channels

TargetOrganism% inhibitionConcentrationRef
Nav1.2H. sapiens85+/-51uMMarkgraf,R. et al. (2012)
Nav1.4H. sapiens60+/-101uMMarkgraf,R. et al. (2012)
95saturationMarkgraf,R. et al. (2012)
84+/-310uMMarkgraf,R. et al. (2012)
R. norvegicus95saturationMarkgraf,R. et al. (2012)
Nav1.5H. sapiens131uMMarkgraf,R. et al. (2012)
610uMMarkgraf,R. et al. (2012)
Nav1.7H. sapiens64+/-51uMMarkgraf,R. et al. (2012)
82+/-310uMMarkgraf,R. et al. (2012)
Nav1.8H. sapiens5+/-51uMMarkgraf,R. et al. (2012)
010uMMarkgraf,R. et al. (2012)

References
Biass,D., Dutertre,S., Gerbault,A., Menou,J.L., Offord,R., Favreau,P. and Stöcklin,R. (2009) Comparative proteomic study of the venom of the piscivorous cone snail Conus consors. J Proteomics 72:210-218
Benoit,E., Favreau,P., Schlumberger,S., Cordova,M.A., Tytgat,J., Stocklin, R. abd and R. Stocklin and Molgo J. (2008) A new mu-conotoxin from Conus consors that atypically targets sodium channels in unmyelinated and myelinated nerve fibers Abstract Book, 16th European Section Meeting of the International Society on Toxinology
Favreau P, Benoit E, Hocking HG, Carlier L, D hoedt D, Leipold E, Markgraf R, Schlumberger S, Córdova MA, Gaertner H, Paolini-Bertrand M, Hartley O, Tytgat J, Heinemann SH, Bertrand D, Boelens R, Stöcklin R, Molgó J (2012) A novel µ-conopeptide, CnIIIC, exerts potent and preferential inhibition of NaV1.2/1.4 channels and blocks neuronal nicotinic acetylcholine receptors Br J Pharmacol 166:1654-1668
Violette,A., Biass,D., Dutertre,S., Koua,D., Piquemal,D., Pierrat,F. and Favreau,P. (2012) Large-scale discovery of conopeptides and conoproteins in the injectable venom of a fish-hunting cone snail using a combined proteomic and transcriptomic approach. J Proteomics 75:5215-5225
Markgraf,R., Leipold,E., Schirmeyer,J., Paolini-Bertrand,M., Hartley,O. and Heinemann,S.H. (2012) Mechanism and molecular basis for the sodium channel subtype specificity of µ-conopeptide CnIIIC. Br. J. Pharmacol. 167:576-586

Internal links
Protein Precursor CnIIIC precursor (6155)
Nucleic acids
Structure Solution structure of the skeletal muscle and neuronal voltage gated sodium channel antagonist mu-conotoxin CnIIIC

External links
UniProtKB/Swiss-Prot I1SB07
Ncbi I1SB07, 2YEN_A

Tools