RIIIJ (P03906) Protein Card

General Information
Name RIIIJ
Alternative name(s) R3J
Organism Conus radiatus
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Notes Cordeiro et al. 2019 reported that RIIIJ blocks heteromeric voltage-gated potassium channels containing the Kv1.2 α-subunits and other subunits from the Kv1 family (Kv1.x).

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework III
Pharmacological family kappa conotoxin

Sequence
LOOCCTOOKKHCOAOACKYKOCCKS
Modified residues
positionsymbolname
2O4-Hydroxyproline
3O4-Hydroxyproline
7O4-Hydroxyproline
8O4-Hydroxyproline
13O4-Hydroxyproline
15O4-Hydroxyproline
21O4-Hydroxyproline
Sequence evidence protein level
Average Mass 2807.29
Monoisotopic Mass 2805.19
Isoelectric Point 11.70
Extinction Coefficient [280nm] 1490.00

Activity

IC50: Voltage-Gated Potassium Channels

TargetOrganismIC50Ref
Kv1.1H. sapiens2.8 μM+/-0.9Cordeiro,S. et al. (2019)
Kv1.2H. sapiens0.32 μM+/-0.08Cordeiro,S. et al. (2019)
Kv1.3H. sapiens1.96 μM+/-0.42Cordeiro,S. et al. (2019)
Kv1.6H. sapiens4.4 μM+/-1.1Cordeiro,S. et al. (2019)
Kv1.7H. sapiens7.41 μM+/-1.41Cordeiro,S. et al. (2019)

Synthetic variants
RIIIJΔ6-11LOOCCSLNLRLCOAOACKYKOCCKS
RIIIJΔ6-8LOOCCSLNKKHCOAOACKYKOCCKS
RIIIJΔ9-11LOOCCTOOLRLCOAOACKYKOCCKS

References
Chen,P., Dendorfer,A., Finol-Urdaneta,R.K., Terlau,H. and Olivera,B.M. (2010) Biochemical characterization of kM-RIIIJ, a KV1.2 channel blocker: Evaluation of cardioprotective effects of kM-conotoxins. J Biol Chem 285:14882-14889
Cordeiro,S., Finol-Urdaneta,R.K., Köpfer,D., Markushina,A., Song,J., French,R.J., Kopec,W., de Groot,B.L., Giacobassi,M.J., Leavitt,L.S., Raghuraman,S., Teichert,R.W., Olivera,B.M. and Terlau,H (2019) Conotoxin κM-RIIIJ, a tool targeting asymmetric heteromeric Kv1 channels Proceedings of the National Academy of Sciences 116:1059-1064

Internal links
Nucleic acids
Structure

External links
Ncbi P0CG45

Tools