RDP-MII [E11R] (P04320) Protein Card

General Information
Name RDP-MII [E11R]
Organism synthetic construct
Protein Type Synthetic
Parent MII

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
RDPGCCSNPVCHLRHSNLC(nh2)
Modified residues
positionsymbolname
20nh2C-term amidation
Average Mass 2106.40
Monoisotopic Mass 2104.89
Isoelectric Point 10.61
Extinction Coefficient [280nm] NA

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α3β2H. sapiens20.1nM+/-5.51uM nicotinePucci,L. et al. (2011)
R. norvegicus33.5nM+/-1.91uM nicotinePucci,L. et al. (2011)
a7*α6β2*R. norvegicus660pM1uM nicotinePucci,L. et al. (2011)

Ki: Nicotinic acetylcholine receptors

TargetOrganismKiCompetitorAgonistRef
α3β2H. sapiens(HEK cell)6.0uM0.1nM epibatidinePucci,L. et al. (2011)
(oocyte)2.7uM0.1nM epibatidinePucci,L. et al. (2011)
R. norvegicus(oocyte)3.1uM0.1nM epibatidinePucci,L. et al. (2011)
α3β2*R. norvegicus4.4uM0.1nM epibatidinePucci,L. et al. (2011)
a7*α6β2*R. norvegicus(Low affinity site)9.2uM0.1nM epibatidinePucci,L. et al. (2011)
(High affinity site)900pM0.1nM epibatidinePucci,L. et al. (2011)

References
Pucci,L., Grazioso,G., Dallanoce,C., Rizzi,L., De Micheli,C., Clementi,F., Bertrand,S., Bertrand,D., Longhi,R., De Amici,M. and Gotti,C. (2011) Engineering of α-conotoxin MII-derived peptides with increased selectivity for native α6β2* nicotinic acetylcholine receptors. FASEB J. 25:3775-3789

Internal links
Nucleic acids
Structure

External links

Tools