PnIA [P6betPro] (P04435) Protein Card

General Information
Name PnIA [P6betPro]
Organism synthetic construct
Protein Type Synthetic
Parent PnIA

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
GCCSL(bet-Pro)PCAANNPD(sTy)C(nh2)
Modified residues
positionsymbolname
6bet-Pro4-(R)-betainamidyl-proline
15sTySulfotyrosine
17nh2C-term amidation
Average Mass 1773.93
Monoisotopic Mass 1772.56
Isoelectric Point 7.00
Extinction Coefficient [280nm] 1490.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α3β4R. norvegicus>300uM300nM epibatidineArmishaw,C. et al. (2009)
α7H. sapiens~300uM30uM AchArmishaw,C. et al. (2009)

Ki: Nicotinic acetylcholine receptors

TargetOrganismKiCompetitorAgonistRef
α3β4R. norvegicus>100uM25 pM 3H-epibatidine5mM S-nicotineArmishaw,C. et al. (2009)
α7-5HT3 chimaeraR. norvegicus~100uM0.5nM 3H-methylcaconitine 5mM S-nicotineArmishaw,C. et al. (2009)

References
Armishaw,C., Jensen,A.A., Balle,T., Clark,R.J., Harpsøe,K., Skonberg,C., Liljefors,T. and Strømgaard,K. (2009) Rational design of alpha-conotoxin analogues targeting alpha7 nicotinic acetylcholine receptors: improved antagonistic activity by incorporation of proline derivatives. J. Biol. Chem. 284:9498-9512

Internal links
Nucleic acids
Structure

External links

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