PnIA [P6fluo(S)Pro] (P04437) Protein Card

General Information
Name PnIA [P6fluo(S)Pro]
Organism synthetic construct
Protein Type Synthetic
Parent PnIA

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
GCCSL(F-(S)-Pro)PCAANNPD(sTy)C(nh2)
Modified residues
positionsymbolname
6F-(S)-Pro4-(S)-fluoro-proline
15sTySulfotyrosine
17nh2C-term amidation
Average Mass 1719.85
Monoisotopic Mass 1718.52
Isoelectric Point 2.19
Extinction Coefficient [280nm] 1490.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α3β4R. norvegicus>300uM300nM epibatidineArmishaw,C. et al. (2009)
α7H. sapiens30-100uM30uM AchArmishaw,C. et al. (2009)

Ki: Nicotinic acetylcholine receptors

TargetOrganismKiCompetitorAgonistRef
α3β4R. norvegicus>100uM25 pM 3H-epibatidine5mM S-nicotineArmishaw,C. et al. (2009)
α7-5HT3 chimaeraR. norvegicus3.7uM0.5nM 3H-methylcaconitine5mM S-nicotineArmishaw,C. et al. (2009)

References
Armishaw,C., Jensen,A.A., Balle,T., Clark,R.J., Harpsøe,K., Skonberg,C., Liljefors,T. and Strømgaard,K. (2009) Rational design of alpha-conotoxin analogues targeting alpha7 nicotinic acetylcholine receptors: improved antagonistic activity by incorporation of proline derivatives. J. Biol. Chem. 284:9498-9512

Internal links
Nucleic acids
Structure

External links

Tools