AuIB [ribbon] (P04480) Protein Card

General Information
Name AuIB [ribbon] (named by ConoServer)
Alternative name(s) rAuIB
Organism synthetic construct
Protein Type Synthetic
Parent AuIB

Classification
Conopeptide class conotoxin
Gene superfamily
Pharmacological family alpha conotoxin

Sequence
GCCSYPPCFATNPDC(nh2)
Modified residues
positionsymbolname
16nh2C-term amidation
Average Mass 1572.75
Monoisotopic Mass 1571.53
Isoelectric Point 5.47
Extinction Coefficient [280nm] 1490.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α3β4R. norvegicus(a3b4 ratio 10:1)860nM0.9450uM AchGrishin,A.A. et al. (2010)
(a3b4 ratio 1:10)NA3uMGrishin,A.A. et al. (2010)
27.5 uM1.1100 μM AChNicke,A. et al. (2003)

Percentage inhibition: Nicotinic acetylcholine receptors

TargetOrganism% inhibitionConcentrationAgonistRef
α3β4R. norvegicus45+/-210uM50-100uM AchLovelace,E.S. et al. (2011)
35Ach (1 uM)Wu et al. (2018)
50Ach (100 uM)Wu et al. (2018)

Synthetic variants
AuIB [N12A,ribbon]GCCSYPPCFATAPDC(nh2)
AuIB[D14A,ribbon]GCCSYPPCFATNPAC(nh2)
AuIB[F9A,ribbon]GCCSYPPCAATNPDC(nh2)
AuIB[G1A,ribbon]ACCSYPPCFATNPDC(nh2)
AuIB[P13A,ribbon]GCCSYPPCFATNADC(nh2)
AuIB[P6A,ribbon]GCCSYAPCFATNPDC(nh2)
AuIB[P7A,ribbon]GCCSYPACFATNPDC(nh2)
AuIB[S4A,ribbon]GCCAYPPCFATNPDC(nh2)
AuIB[T11A,ribbon]GCCSYPPCFAANPDC(nh2)
AuIB[Y5A,ribbon]GCCSAPPCFATNPDC(nh2)

References
Lovelace,E.S., Gunasekera,S., Alvarmo,C., Clark,R.J., Nevin,S.T., Grishin,A.A., Adams,D.J., Craik,D.J., and Daly,N.L. (2011) Stabilization of α-conotoxin AuIB: influences of disulfide connectivity and backbone cyclization. Antioxid. Redox Signal. 14:87-95
Grishin,A.A., Wang,C.I., Muttenthaler,M., Alewood,P.F., Lewis,R.J. and Adams,D.J. (2010) Alpha-conotoxin AuIB isomers exhibit distinct inhibitory mechanisms and differential sensitivity to stoichiometry of alpha3beta4 nicotinic acetylcholine receptors. J. Biol. Chem. 285:22254-22263
Nicke,A., Samochocki,M., Loughnan,M.L., Bansal,P.S., Maelicke,A. and Lewis,R.J. (2003) Alpha-conotoxins EpI and AuIB switch subtype selectivity and activity in native versus recombinant nicotinic acetylcholine receptors. FEBS Lett. 554:219-223
Grishin,A.A., Cuny,H., Hung,A., Clark,R.J., Brust,A., Akondi,K., Alewood,P.F., Craik,D.J. and Adams,D.J. (2013) Identifying key amino acid residues that affect α-conotoxin AuIB inhibition of α3β4 nicotinic acetylcholine receptors. J. Biol. Chem. 288:34428-34442
Wu, X., Tae, H.S., Huang, Y.H., Adams, D.J., Craik, D.J. and Kaas, Q. (2018) Stoichiometry dependent inhibition of rat α3β4 nicotinic acetylcholine receptor by the ribbon isomer of α-conotoxin AuIB Biochemical pharmacology 155:288-297

Internal links
Nucleic acids
Structure SOLUTION STRUCTURE OF THE RIBBON DISULFIDE BOND ISOMER OF ALPHA-CONOTOXIN AUIB

External links

Tools