AuIB [ribbon isoform] (P04480) Protein Card

General Information
Name AuIB [ribbon isoform]
Organism synthetic construct
Protein Type Synthetic
Parent AuIB

Classification
Conopeptide class conotoxin
Gene superfamily
Pharmacological family alpha conotoxin

Sequence
GCCSYPPCFATNPDC(nh2)
Modified residues
positionsymbolname
16nh2C-term amidation
Average Mass 1572.75
Monoisotopic Mass 1571.53
Isoelectric Point 5.47
Extinction Coefficient [280nm] 1490.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α3β4R. norvegicus(a3b4 ratio 10:1)860nM0.9450uM AchGrishin,A.A. et al. (2010)
(a3b4 ratio 1:10)NA3uMGrishin,A.A. et al. (2010)
27.5 uM1.1100 μM AChNicke,A. et al. (2003)

Percentage inhibition: Nicotinic acetylcholine receptors

TargetOrganism% inhibitionConcentrationAgonistRef
α3β4R. norvegicus45+/-210uM50-100uM AchLovelace,E.S. et al. (2011)

References
Lovelace,E.S., Gunasekera,S., Alvarmo,C., Clark,R.J., Nevin,S.T., Grishin,A.A., Adams,D.J., Craik,D.J., and Daly,N.L. (2011) Stabilization of α-conotoxin AuIB: influences of disulfide connectivity and backbone cyclization. Antioxid. Redox Signal. 14:87-95
Grishin,A.A., Wang,C.I., Muttenthaler,M., Alewood,P.F., Lewis,R.J. and Adams,D.J. (2010) Alpha-conotoxin AuIB isomers exhibit distinct inhibitory mechanisms and differential sensitivity to stoichiometry of alpha3beta4 nicotinic acetylcholine receptors. J. Biol. Chem. 285:22254-22263
Nicke,A., Samochocki,M., Loughnan,M.L., Bansal,P.S., Maelicke,A. and Lewis,R.J. (2003) Alpha-conotoxins EpI and AuIB switch subtype selectivity and activity in native versus recombinant nicotinic acetylcholine receptors. FEBS Lett. 554:219-223

Internal links
Nucleic acids
Structure SOLUTION STRUCTURE OF THE RIBBON DISULFIDE BOND ISOMER OF ALPHA-CONOTOXIN AUIB

External links

Tools