GeXIVA[ribbon] (P05344) Protein Card

General Information
Name GeXIVA[ribbon] (named by ConoServer)
Alternative name(s) Ge14a,GeXIVA[1-4]
Organism Conus generalis
Organism region Indo-Pacific
Organism diet vermivorous
Protein Type Wild type
Protein precursor GeXIVA precursor (5224)
Notes GeXIVA inhibits Sf9 cell growth (Gao 2013). There is a discrepancy in the sequence initally published in GenBank in 2012 and the sequence published in peer-reviewed journals (involving the same authors).

Classification
Conopeptide class conotoxin
Gene superfamily O1 superfamily
Cysteine framework XIV
Pharmacological family alpha conotoxin

Sequence
TCRSSGRYCRSPYDRRRRYCRRITDACV
Sequence evidence protein level
Average Mass 3452.92
Monoisotopic Mass 3450.62
Isoelectric Point 12.80
Extinction Coefficient [280nm] 4470.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α1β1δεM. musculus840 nM[583-1206]Ach (10 uM)Xu et al. (2020)
α2β2R. norvegicus440 nM[335-575]Ach (100 uM)Xu et al. (2020)
α3β2R. norvegicus1250 nM[782-1991]Ach (100 uM)Xu et al. (2020)
α3β4R. norvegicus3400 nM[1279-9048]Ach (100 uM)Xu et al. (2020)
α6/α3β4R. norvegicus1110 nM[30-1949]Ach (100 uM)Xu et al. (2020)
α7R. norvegicus2830 nM[1910-4188]Ach (200 uM)Xu et al. (2020)
α9α10R. norvegicus7.0 nM[3.6-13.4]0.79Ach (10 uM)Luo,S. et al. (2015)
16 nM[13-20]Ach (10 uM)Xu et al. (2020)
7 nM[3.6-13.4]Ach (10 uM)Luo,S. et al. (2015)

Synthetic variants
GeXIVA[C2A,C27A]TARSSGRYCRSPYDRRRRYCRRITDAAV
GeXIVA[C2S,C27S]TSRSSGRYCRSPYDRRRRYCRRITDASV
GeXIVA[C9A,C20A]TCRSSGRYARSPYDRRRRYARRITDACV
GeXIVA[C9S,C20S]TCRSSGRYSRSPYDRRRRYSRRITDACV

References
Gao,B., Zhangsun,D., Wu,Y., Lin,B., Zhu,X. and Luo,S. (2013) Expression, renaturation and biological activity of recombinant conotoxin GeXIVAWT. Appl. Microbiol. Biotechnol. 97:1223-1230
Luo,S., Zhangsun,D., Harvey,P.J., Kaas,Q., Wu,Y., Zhu,X., Hu,Y., Li,X., Tsetlin,V.I., Christensen,S., Romero,H.K., McIntyre,M., Dowell,C., Baxter,J.C., Elmslie,K.S., Craik,D.J. and McIntosh,J.M. (2015) Cloning, synthesis, and characterization of αO-conotoxin GeXIVA, a potent α9α10 nicotinic acetylcholine receptor antagonist. Proc. Natl. Acad. Sci. U.S.A. 112-35
Wang, H., Li, X., Zhangsun, D., Yu, G., Su, R. and Luo, S. (2019) The α9α10 Nicotinic Acetylcholine Receptor Antagonist αO-Conotoxin GeXIVA[1,2] Alleviates and Reverses Chemotherapy-Induced Neuropathic Pain Marine drugs 17:265
Xu, P., Kaas, Q., Wu, Y., Zhu, X., Li, X., Harvey, P.J., Zhangsun, D., Craik, D.J. and Luo, S (2020) Structure and Activity Studies of Disulfide-Deficient Analogues of αO-Conotoxin GeXIVA Journal of Medicinal Chemistry

Internal links
Protein Precursor GeXIVA precursor (5224)
Nucleic acids
Structure

External links

Tools