TxIB (P05345) Protein Card

General Information
Name TxIB
Organism Conus textile (Cloth-of-gold cone)
Organism region Indo-Pacific
Organism diet molluscivorous
Protein Type Wild type
Protein precursor TxIB precursor (5225)
Notes You et al.,2019 injected TxIB in mice and reported the behaviour of the animal: TxIB inhibited the NIC-induced conditioned place preference expression and reinstatement with a dose-dependent manner. It had no significant effect on locomotor activity after intracerebroventricular injection in mice.

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
GCCSDPPCRNKHPDLC(nh2)
Modified residues
positionsymbolname
17nh2C-term amidation
Sequence evidence nucleic acid level
Average Mass 1739.97
Monoisotopic Mass 1738.68
Isoelectric Point 8.31
Extinction Coefficient [280nm] -1.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α1β1δεM. musculus>10uM10uM AChLuo,S. et al. (2013)
α2β2R. norvegicus>10uM100uM AChLuo,S. et al. (2013)
α2β4R. norvegicus>10uM100uM AChLuo,S. et al. (2013)
α3β2R. norvegicus>10uM100uM AChLuo,S. et al. (2013)
α3β4R. norvegicus >10uM100uM AChLuo,S. et al. (2013)
α4β2R. norvegicus >10uM100uM AChLuo,S. et al. (2013)
α4β4R. norvegicus >10uM100uM AChLuo,S. et al. (2013)
α6/α3β2β3H. sapiens291 nM[255-332]100uM AChHone et al. (2018)
R. norvegicus207 nM[175-244]100uM AChHone et al. (2018)
28.4nM+/-15100uM AChLuo,S. et al. (2013)
41.7nM[32.1-54.22]100uM AChYu,J. et al. (2018)
α6/α3β4H. sapiens402 nM[338-477] 300uM AchHone et al. (2018)
R. norvegicus>10uM100uM AChLuo,S. et al. (2013)
>10 uM300uM AchHone et al. (2018)
α7R. norvegicus>10uM200uM AChLuo,S. et al. (2013)
α9α10R. norvegicus>10uM10uM AChLuo,S. et al. (2013)

Percentage inhibition: Nicotinic acetylcholine receptors

TargetOrganism% inhibitionConcentrationAgonistRef
α6β2β3M. musculus42100 nMAch (100 uM)Li,X. et al. (2020)

Synthetic variants
TxIB[K11A]GCCSDPPCRNAHPDLC(nh2)
TxIB[insC_G]GCCSDPPCRNKHPDLCG
TxIB[insC_M]GCCSDPPCRNKHPDLCM
TxIB[insN_GAAG] cyclicGAAGGCCSDPPCRNKHPDLC
TxIB[insN_GAGAGAAG] cyclicGAGAAGGCCSDPPCRNKHPDLC
TxIB[insN_GGAAGAG] cyclicGGAAGAGGCCSDPPCRNKHPDLC
TxIB[insN_GGAAG] cyclicGGAAGGCCSDPPCRNKHPDLC

References
Luo,S., Zhangsun,D., Wu,Y., Zhu,X., Hu,Y., McIntyre,M., Christensen,S., Akcan,M., Craik,D.J. and McIntosh,J.M. (2013) Characterization of a novel α-conotoxin from conus textile that selectively targets α6/α3β2β3 nicotinic acetylcholine receptors. J. Biol. Chem. 288:894-902
Hone, A.J., Talley, T.T., Bobango, J., Melo, C.H., Hararah, F., Gajewiak, J., Christensen, S., Harvey, P.J., Craik, D.J. and McIntosh, J.M. (2018) Molecular determinants of α-conotoxin potency for inhibition of human and rat α6β4 nicotinic acetylcholine receptors Journal of Biological Chemistry 293:17838-17852
Li,X., Wang,S., Zhu,X., Zhangsun,D., Wu,Y. and Luo,S (2020) Effects of Cyclization on Activity and Stability of α-Conotoxin TxIB Mar Drugs 18
You,S., Li,X., Xiong,J., Zhu,X., Zhangsun,D., Zhu,X. and Luo,S. (2019) α-Conotoxin TxIB: A Uniquely Selective Ligand for α6/α3β2β3 Nicotinic Acetylcholine Receptor Attenuates Nicotine-Induced Conditioned Place Preference in Mice Mar Drugs 17:490
Yu,J., Zhu,X., Yang,Y., Luo,S. and Zhangsun,D. (2018) Expression in Escherichia coli of fusion protein comprising α-conotoxin TxIB and preservation of selectivity to nicotinic acetylcholine receptors in the purified product Chem Biol Drug Des 91:349-358

Internal links
Protein Precursor TxIB precursor (5225)
Nucleic acids
Structure Solution structure of a Novel Alpha-Conotoxin TxIB

External links

Tools