LsIA (P06776) Protein Card

General Information
Name LsIA
Organism Conus limpusi
Organism region Indo-Pacific
Organism diet vermivorous
Protein Type Wild type

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
SGCCSNPACRVNNPNIC(nh2)
Modified residues
positionsymbolname
18nh2C-term amidation
Sequence evidence protein level
Average Mass 1746.96
Monoisotopic Mass 1745.68
Isoelectric Point 10.94
Extinction Coefficient [280nm] NA

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α3α5β2R. norvegicus31.2 nM+/-5.6-1.150 uM AchInserra,M.C. et al. (2013)
α3β2R. norvegicus10.3 nM+/-1.6-2.150 uM AchInserra,M.C. et al. (2013)
α7H. sapiens10.1 nM+/-1.4-1.3200 uM AchInserra,M.C. et al. (2013)

Ki: Nicotinic acetylcholine receptors

TargetOrganismKiCompetitorAgonistRef
α3α5β2R. norvegicus9.6 nMAchInserra,M.C. et al. (2013)
α3β2R. norvegicus15.2 nMAchInserra,M.C. et al. (2013)
α7H. sapiens1.47 nMAchInserra,M.C. et al. (2013)

Percentage inhibition: Nicotinic acetylcholine receptors

TargetOrganism% inhibitionConcentrationAgonistRef
α3β4R. norvegicus40+/-53 uM50 uM AchInserra,M.C. et al. (2013)
α4β2R. norvegicus03 uM50 uM AchInserra,M.C. et al. (2013)
α4β4R. norvegicus03 uM50 uM AchInserra,M.C. et al. (2013)
α9α10R. norvegicus03 uM50 uM AchInserra,M.C. et al. (2013)

Synthetic variants
LsIA [Δ1-2]CCSNPACRVNNPNIC(nh2)
LsIA [Δ1]GCCSNPACRVNNPNIC(nh2)
LsIA#SGCCSNPACRVNNPNIC

References
Inserra,M.C., Kompella,S.N., Vetter,I., Brust,A., Daly,N.L., Cuny,H., Craik,D.J., Alewood,P.F., Adams,D.J. and Lewis,R.J. (2013) Isolation and characterization of α-conotoxin LsIA with potent activity at nicotinic acetylcholine receptors. Biochem. Pharmacol. 86:791-799

Internal links
Nucleic acids
Structure Structural mechanisms for alpha-conotoxin selectivity at the human alpha3beta4 nicotinic acetylcholine receptor

External links

Tools