TxVC (P06814) Protein Card

General Information
Name TxVC
Organism Conus textile (Cloth-of-gold cone)
Organism region Indo-Pacific
Organism diet molluscivorous
Protein Type Wild type
Notes Wand et al. claim this peptide belongs to the T-superfamily based on the cysteine framework, but they did not isolate the corresponding gene or mRNA. Without the sequence of the precusor, it cannot be unambiguously stated that this peptide is produced from a T-superfamily gene. TxVC is therefore potentially the first T-superfamily conotoxin that inhibits nAChRs (Wang et al., 2014).

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework V
Pharmacological family alpha conotoxin

Sequence
KPCCSIHDNSCCGL
Sequence evidence protein level
Average Mass 1475.68
Monoisotopic Mass 1474.55
Isoelectric Point 7.98
Extinction Coefficient [280nm] NA

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α2β2C. textile>10000 nMACh (100 uM)Wang,S. et al. (2014)
α2β4C. textile>10000 nMACh (100 uM)Wang,S. et al. (2014)
α3β2C. textile1032.8 nMACh (100 uM)Wang,S. et al. (2014)
α3β4C. textile>10000 nMACh (100 uM)Wang,S. et al. (2014)
α4β2C. textile343.4 nMACh (100 uM)Wang,S. et al. (2014)
α4β4C. textile>10000 nMACh (100 uM)Wang,S. et al. (2014)
α7C. textile>10000 nMACh (200 uM)Wang,S. et al. (2014)
α9α10C. textile>10000 nMACh (30 uM)Wang,S. et al. (2014)

Synthetic variants
TxVC[H7A]LGCCSNDAISCCPK
TxVC[I6A]LGCCSNDHASCCPK
TxVC[K1A]LGCCSNDHISCCPA
TxVC[L14A]AGCCSNDHISCCPK
TxVC[P2A]LGCCSNDHISCCAK

References
Wang,S., Du,T., Liu,Z., Wang,S., Wu,Y., Ding,J., Jiang,L. and Dai,Q. (2014) Characterization of a T-superfamily conotoxin TxVC from Conus textile that selectively targets neuronal nAChR subtypes. Biochem. Biophys. Res. Commun. 454:151-156

Internal links
Nucleic acids
Structure

External links

Tools