GVIIJ (P06822) Protein Card

General Information
Name GVIIJ
Alternative name(s) μO§-GVIIJ
Organism Conus geographus (Geography cone)
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor GVIIJ precursor (5732)

Classification
Conopeptide class conotoxin
Gene superfamily O1 superfamily
Cysteine framework VI/VII
Pharmacological family mu conotoxin

Sequence
G(Btr)CGDOGATCGKLRLYCCSGFCD(Scc)YTKTCKDKS
SA
Modified residues
positionsymbolname
2BtrBromotryptophan
6O4-Hydroxyproline
24SccS-cysteinylated cysteine
Sequence evidence protein level
Average Mass 3937.25
Monoisotopic Mass 3933.41
Isoelectric Point 10.68
Extinction Coefficient [280nm] 2980.00

Activity

Kd: Voltage-Gated Sodium Channels

TargetOrganismKdRef
Nav1.2R. norvegicus0.0029 μM+/-0.0004Gajewiak,J. et al. (2014)
Nav1.7R. norvegicus0.0032 μM+/-0.0013Gajewiak,J. et al. (2014)

Synthetic variants
GVIIJ covalent dimerGWCGDOGATCGKLRLYCCSGFCDCYTKTCKDKSSA
GVIIJ[(Btr)2A,(Scc)24(Eac)]GACGDOGATCGKLRLYCCSGFCD(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24(Eac),D31K]GWCGDOGATCGKLRLYCCSGFCD(Eac)YTKTCKKKSSA
GVIIJ[(Btr)2W,(Scc)24(Eac),K27D]GWCGDOGATCGKLRLYCCSGFCD(Eac)YTDTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24(Eac),K27F]GWCGDOGATCGKLRLYCCSGFCD(Eac)YTFTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24(Eac),K27G]GWCGDOGATCGKLRLYCCSGFCD(Eac)YTGTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24(Eac),K32D]GWCGDOGATCGKLRLYCCSGFCD(Eac)YTKTCKDDSSA
GVIIJ[(Btr)2W,(Scc)24(Eac),Y25A]GWCGDOGATCGKLRLYCCSGFCD(Eac)ATKTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24(Eac),Y25D]GWCGDOGATCGKLRLYCCSGFCD(Eac)DTKTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24(Eac),Y25R]GWCGDOGATCGKLRLYCCSGFCD(Eac)RTKTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24(Eac)]GWCGDOGATCGKLRLYCCSGFCD(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24(Msc)]GWCGDOGATCGKLRLYCCSGFCD(Msc)YTKTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24(Sgc)]GWCGDOGATCGKLRLYCCSGFCD(Sgc)YTKTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24(Smc)]GWCGDOGATCGKLRLYCCSGFCD(Smc)YTKTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24(Tcc)]GWCGDOGATCGKLRLYCCSGFCD(Tcc)YTKTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24C,T26A]GWCGDOGATCGKLRLYCCSGFCDCYAKTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24C,T28A]GWCGDOGATCGKLRLYCCSGFCDCYTKACKDKSSA
GVIIJ[(Btr)2W,(Scc)24C]GWCGDOGATCGKLRLYCCSGFCDCYTKTCKDKSSA
GVIIJ[(Btr)2W,(Scc)24S]GWCGDOGATCGKLRLYCCSGFCDSYTKTCKDKSSA
GVIIJ[(Btr)2W,D23(Gla),(Scc)24(Eac)]GWCGDOGATCGKLRLYCCSGFC(Gla)(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W,D23N,(Scc)24(Eac)]GWCGDOGATCGKLRLYCCSGFCN(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W,D5K,(Scc)24(Eac)]GWCGKOGATCGKLRLYCCSGFCD(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W,F21A,(Scc)24C]GWCGDOGATCGKLRLYCCSGACDCYTKTCKDKSSA
GVIIJ[(Btr)2W,K12D,(Scc)24(Eac)]GWCGDOGATCGDLRLYCCSGFCD(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W,L13A,(Scc)24(Eac)]GWCGDOGATCGKARLYCCSGFCD(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W,L15A,(Scc)24(Eac)]GWCGDOGATCGKLRAYCCSGFCD(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W,O6A,(Scc)24(Eac)]GWCGDAGATCGKLRLYCCSGFCD(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W,R14D,(Scc)24(Eac)]GWCGDOGATCGKLDLYCCSGFCD(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W,S19A,(Scc)24(Eac)]GWCGDOGATCGKLRLYCCAGFCD(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W,T9A,(Scc)24(Eac)]GWCGDOGAACGKLRLYCCSGFCD(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W,Y16A,(Scc)24(Eac)]GWCGDOGATCGKLRLACCSGFCD(Eac)YTKTCKDKSSA
GVIIJ[(Btr)2W]GWCGDOGATCGKLRLYCCSGFCD(Scc)YTKTCKDKSSA

References
Gajewiak,J., Azam,L., Imperial,J., Walewska,A., Green,B.R., Bandyopadhyay,P.K., Raghuraman,S., Ueberheide,B., Bern,M., Zhou,H.M., Minassian,N.A., Hagan,R.H., Flinspach,M., Liu,Y., Bulaj,G., Wickenden,A.D., Olivera,B.M., Yoshikami,D. and Zhang,M.M. (2014) A disulfide tether stabilizes the block of sodium channels by the conotoxin μO§-GVIIJ. Proc. Natl. Acad. Sci. U.S.A. 111:2758-2763

Internal links
Protein Precursor GVIIJ precursor (5732)
Nucleic acids
Structure NMR solution structure: GVIIJ[C24S]

External links

Tools