GVIIJ (P06822) Protein Card

General Information
Name GVIIJ
Alternative name(s) μO§-GVIIJ
Organism Conus geographus (Geography cone)
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor GVIIJ precursor (5732)

Classification
Conopeptide class conotoxin
Gene superfamily O1 superfamily
Cysteine framework VI/VII
Pharmacological family mu conotoxin

Sequence
G(BTr)CGDOGATCGKLRLYCCSGFCD(SCC)YTKTCKDKS
SA
Modified residues
positionsymbolname
2BTrBromotryptophan
6O4-Hydroxyproline
24SCCS-cysteinylated cysteine
Sequence evidence protein level
Average Mass 3937.25
Monoisotopic Mass 3933.41
Isoelectric Point 10.68
Extinction Coefficient [280nm] 2980.00

Activity

Kd: Voltage-Gated Sodium Channels

TargetOrganismKdRef
Nav1.2R. norvegicus0.0029 μM+/-0.0004Gajewiak,J. et al. (2014)
Nav1.7R. norvegicus0.0032 μM+/-0.0013Gajewiak,J. et al. (2014)

Synthetic variants
(GVIIJ)2ASSKDKCTKTYCDCFGSCCYLRLKGCTAGODGCWG
GVIIJ[BTr2W, SCC24C]ASSKDKCTKTYCDCFGSCCYLRLKGCTAGODGCWG
GVIIJ[BTr2W, SCC24EAC]ASSKDKCTKTY(EAC)DCFGSCCYLRLKGCTAGODGCWG
GVIIJ[BTr2W, SCC24MSC]ASSKDKCTKTY(MSC)DCFGSCCYLRLKGCTAGODGCWG
GVIIJ[BTr2W, SCC24SGC]ASSKDKCTKTY(SGC)DCFGSCCYLRLKGCTAGODGCWG
GVIIJ[BTr2W, SCC24SMC]ASSKDKCTKTY(SMC)DCFGSCCYLRLKGCTAGODGCWG
GVIIJ[BTr2W, SCC24S]ASSKDKCTKTYCDCFGSCCYLRLKGCTAGODGCWG
GVIIJ[BTr2W, SCC24TCC]ASSKDKCTKTY(TCC)DCFGSCCYLRLKGCTAGODGCWG
GVIIJ[BTr2W]ASSKDKCTKTY(SCC)DCFGSCCYLRLKGCTAGODGCWG

References
Gajewiak,J., Azam,L., Imperial,J., Walewska,A., Green,B.R., Bandyopadhyay,P.K., Raghuraman,S., Ueberheide,B., Bern,M., Zhou,H.M., Minassian,N.A., Hagan,R.H., Flinspach,M., Liu,Y., Bulaj,G., Wickenden,A.D., Olivera,B.M., Yoshikami,D. and Zhang,M.M. (2014) A disulfide tether stabilizes the block of sodium channels by the conotoxin μO§-GVIIJ. Proc. Natl. Acad. Sci. U.S.A. 111:2758-2763

Internal links
Protein Precursor GVIIJ precursor (5732)
Nucleic acids
Structure NMR solution structure: GVIIJ[C24S]

External links

Tools