GVIIJ[(Btr)2W] (P06835) Protein Card

General Information
Name GVIIJ[(Btr)2W] (named by ConoServer)
Alternative name(s) GVIIJ[SSC]
Organism
Protein Type Synthetic
Parent GVIIJ
Notes

No activity against mouse Nav 1.6 if co-expressed with β2 or β4 subunit [Wilson, M.J., Zhang, M. (2015)].

Does not block A-fiber or C-fiber compound action potentials in mouse and rat sciatic nerves [Wilson, M.J., Zhang, M. (2015)].


Classification
Conopeptide class conotoxin
Gene superfamily O1 superfamily
Cysteine framework VI/VII
Pharmacological family mu conotoxin

Sequence
GWCGDOGATCGKLRLYCCSGFCD(Scc)YTKTCKDKSSA
Modified residues
positionsymbolname
6O4-Hydroxyproline
24SccS-cysteinylated cysteine
Sequence evidence protein level
Average Mass 3858.35
Monoisotopic Mass 3855.50
Isoelectric Point 10.68
Extinction Coefficient [280nm] 8480.00

Activity

Kd: Voltage-Gated Sodium Channels

TargetOrganismKdRef
Nav1.2R. norvegicus(Expressed in X. laevis oocytes)0.0034 μM+/-0.0013Gajewiak,J. et al. (2014)
Nav1.6M. musculus(With β3 subunit. Expressed in X. laevis oocytes.)0.055 μM+/-0.015Wilson,M.J. et al. (2015)
(With β1 subunit. Expressed in X. laevis oocytes.)0.029 μM+/-0.016Wilson,M.J. et al. (2015)
(Expressed in X. laevis oocytes.)0.06 μM+/-0.023Wilson,M.J. et al. (2015)
Nav1.7R. norvegicus(Expressed in X. laevis oocytes)0.013 μM+/-0.006Gajewiak,J. et al. (2014)

References
Gajewiak,J., Azam,L., Imperial,J., Walewska,A., Green,B.R., Bandyopadhyay,P.K., Raghuraman,S., Ueberheide,B., Bern,M., Zhou,H.M., Minassian,N.A., Hagan,R.H., Flinspach,M., Liu,Y., Bulaj,G., Wickenden,A.D., Olivera,B.M., Yoshikami,D. and Zhang,M.M. (2014) A disulfide tether stabilizes the block of sodium channels by the conotoxin μO§-GVIIJ. Proc. Natl. Acad. Sci. U.S.A. 111:2758-2763
Wilson,M.J., Zhang,M.M., Gajewiak,J., Azam,L., Rivier,J.E., Olivera,B.M. and Yoshikami,D. (2015) Α- and β-subunit composition of voltage-gated sodium channels investigated with μ-conotoxins and the recently discovered μO§-conotoxin GVIIJ. J. Neurophysiol. 113:2289-2301

Internal links
Nucleic acids
Structure

External links

Tools