ViIA (P06858) Protein Card

General Information
Name ViIA
Alternative name(s) VilA-II
Organism Conus virgo
Organism region Indo-Pacific
Organism diet vermivorous
Protein Type Wild type
Protein precursor VilA precursor (6859)
Notes Only four alpha ono toxins belonging to 4/6 subgroup, including TxID, AuIB, Bu22 and TiLA, have been reported. VilA is the first 4/6 alpha-conotoxin that selectively inhibits nAChR a3b2 subtype ( Li et al. 2015)

Classification
Conopeptide class conotoxin
Gene superfamily A superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
RDCCSNPPCAHNNPDC(nh2)
Modified residues
positionsymbolname
17nh2C-term amidation
Sequence evidence protein level
Average Mass 1740.87
Monoisotopic Mass 1739.60
Isoelectric Point 5.80
Extinction Coefficient [280nm] NA

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α2β2R. norvegicus(No SD or higher and lower brackets provided)>10 uMACh (100 uM)Li,L. et al. (2015)
α2β4R. norvegicus(No SD or higher and lower brackets provided)>10 uMACh (100 uM)Li,L. et al. (2015)
α3β2R. norvegicus845.5 nMACh (100 uM)Li,L. et al. (2015)
α3β4R. norvegicus(No SD or higher and lower brackets provided)>10 uMACh (100 uM)Li,L. et al. (2015)
α4β2R. norvegicus(No SD or higher and lower brackets provided)>10 uMACh (100 uM)Li,L. et al. (2015)
α4β4R. norvegicus(No SD or higher and lower brackets provided)>10 uMACh (100 uM)Li,L. et al. (2015)
α7R. norvegicus(No SD or higher and lower brackets provided)>10 uMACh (200 uM)Li,L. et al. (2015)
α9α10R. norvegicus(No SD or higher and lower brackets provided)>10 uMACh (30 uM)Li,L. et al. (2015)

Synthetic variants
ViIA[R1A]ADCCSNPPCAHNNPDC(nh2)
VilA[D2A]RACCSNPPCAHNNPDC(nh2)
VilA[H11A]RDCCSNPPCAANNPDC(nh2)
VilA[deamidated]RDCCSNPPCAHNNPD(nh2)
VilA[ins15_L]RDCCSNPPCAHNNPDLC(nh2)

References
Li,L., Liu,N., Ding,R., Wang,S., Liu,Z., Li,H., Zheng,X. and Dai,Q. (2015) A novel 4/6-type alpha-conotoxin ViIA selectively inhibits nAchR α3β2 subtype. Acta Biochim. Biophys. Sin. (Shanghai) 47:1023-1028

Internal links
Protein Precursor VilA precursor (6859)
Nucleic acids
Structure

External links

Tools