GVIIJ[(Btr)2W,(Scc)24(Eac)] (P06951) Protein Card

General Information
Name GVIIJ[(Btr)2W,(Scc)24(Eac)] (named by ConoServer)
Alternative name(s) GVIIJ[SSEA]
Organism synthetic construct
Protein Type Synthetic
Parent GVIIJ
Notes Residues in loop 4 important for structural stability, loop 2 for interaction with target (Green, B.R. et al., 2016)

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework VI/VII
Pharmacological family mu conotoxin

Sequence
GWCGDOGATCGKLRLYCCSGFCD(Eac)YTKTCKDKSSA
Modified residues
positionsymbolname
6O4-Hydroxyproline
24Eac 2-aminoethanethiol (cysteamine) cysteine
Sequence evidence protein level
Average Mass 3815.35
Monoisotopic Mass 3812.52
Isoelectric Point 10.68
Extinction Coefficient [280nm] 8480.00

Activity

Kd: Voltage-Gated Sodium Channels

TargetOrganismKdRef
Nav1.2R. norvegicus0.0023 μMZhang,M.M. et al. (2015)
Nav1.6M. musculus0.049 μMZhang,M.M. et al. (2015)

References
Zhang,M.M., Gajewiak,J., Azam,L., Bulaj,G., Olivera,B.M. and Yoshikami,D. (2015) Probing the Redox States of Sodium Channel Cysteines at the Binding Site of μO§-Conotoxin GVIIJ. Biochemistry 54:3911-3920
Green,B.R., Gajewiak,J., Chhabra,S., Skalicky,J.J., Zhang,M.M., Rivier,J.E., Bulaj,G., Olivera,B.M., Yoshikami,D. and Norton,R.S. (2016) Structural Basis for the Inhibition of Voltage-gated Sodium Channels by Conotoxin μO§-GVIIJ. J. Biol. Chem. 291:7205-7220

Internal links
Nucleic acids
Structure NMR chemical shifts: GVIIJ[SCC24EAC]

External links

Tools