GVIIJ[BTr2W, SCC24EAC] (P06951) Protein Card

General Information
Name GVIIJ[BTr2W, SCC24EAC] (named by ConoServer)
Alternative name(s) GVIIJ[SSEA]
Organism
Protein Type Synthetic
Parent GVIIJ
Notes Residues in loop 4 important for structural stability, loop 2 for interaction with target (Green, B.R. et al., 2016)

Classification
Conopeptide class conotoxin
Gene superfamily O1 superfamily
Cysteine framework VI/VII
Pharmacological family mu conotoxin

Sequence
GWCGDOGATCGKLRLYCCSGFCD(EAC)YTKTCKDKSSA
Modified residues
positionsymbolname
6O4-Hydroxyproline
24EAC 2-aminoethanethiol (cysteamine) cysteine
Sequence evidence protein level
Average Mass 3815.35
Monoisotopic Mass 3812.52
Isoelectric Point 10.68
Extinction Coefficient [280nm] 8480.00

Activity

Kd: Voltage-Gated Sodium Channels

TargetOrganismKdRef
Nav1.2R. norvegicus(Expressed in X. laevis oocytes. Kon & Koff rate constants recorded +/- SD in original article.)0.0023 μMZhang,M.M. et al. (2015)
Nav1.6M. musculus(Expressed in X. laevis oocytes. Kon & Koff rate constants recorded +/- SD in original article.)0.049 μMZhang,M.M. et al. (2015)

References
Zhang,M.M., Gajewiak,J., Azam,L., Bulaj,G., Olivera,B.M. and Yoshikami,D. (2015) Probing the Redox States of Sodium Channel Cysteines at the Binding Site of μO§-Conotoxin GVIIJ. Biochemistry 54:3911-3920
Green,B.R., Gajewiak,J., Chhabra,S., Skalicky,J.J., Zhang,M.M., Rivier,J.E., Bulaj,G., Olivera,B.M., Yoshikami,D. and Norton,R.S. (2016) Structural Basis for the Inhibition of Voltage-gated Sodium Channels by Conotoxin μO§-GVIIJ. J. Biol. Chem. 291:7205-7220

Internal links
Nucleic acids
Structure NMR chemical shifts: GVIIJ[SCC24EAC]

External links

Tools