PnIA [L5R,A9R,A10L,D14R] (P07529) Protein Card

General Information
Name PnIA [L5R,A9R,A10L,D14R] (named by ConoServer)
Alternative name(s) PnIA [R5,R9,L10,R14]
Organism synthetic construct
Protein Type Synthetic
Parent PnIA

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
GCCSRPPCRLNNPRYC(nh2)
Modified residues
positionsymbolname
17nh2C-term amidation
Average Mass 1834.14
Monoisotopic Mass 1832.78
Isoelectric Point 12.80
Extinction Coefficient [280nm] 1490.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillCompetitorAgonistRef
α7R. norvegicus0.86uM+/-0.021.4[125I] BgtAch (100 uM)Kasheverov,I.E. et al. (2016)
17nM+/-2Ach (100 uM)Kasheverov,I.E. et al. (2016)
0.39uM+/-0.0751.8[125I] PnIA[R9,L10]Ach (100 uM)Kasheverov,I.E. et al. (2016)
AChBPA. californica24nM+/-20.92[125I] BgtAch (140 nM)Kasheverov,I.E. et al. (2016)
L. stagnalis1.22nM+/-0.042.4[125I] BgtAch (2.4 nM)Kasheverov,I.E. et al. (2016)

References
Kasheverov,I.E., Chugunov,A.O., Kudryavtsev,D.S., Ivanov,I.A., Zhmak,M.N., Shelukhina,I.V., Spirova,E.N., Tabakmakher,V.M., Zelepuga,E.A., Efremov,R.G. and Tsetlin,V.I. (2016) High-Affinity α-Conotoxin PnIA Analogs Designed on the Basis of the Protein Surface Topography Method. Sci Rep 6:36848

Internal links
Nucleic acids
Structure

External links

Tools