MIIIJ (P08892) Protein Card

General Information
Name MIIIJ
Organism Conus magus (Magus cone)
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Notes Rybin et al.,2020 reported that injections of MIIIJ in goldfish (Carassius auratus) induced paralysis but not in mice. They also predict MIIIJ is from M superfamily but lacking precursor data.

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework III
Pharmacological family alpha conotoxin

Sequence
ZKCCSGGSCPLYFRDRLICPCC
Modified residues
positionsymbolname
1ZPyroglutamic acid
Sequence evidence protein level
Average Mass NA
Monoisotopic Mass NA
Isoelectric Point 11.62
Extinction Coefficient [280nm] 1490.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50AgonistRef
α1β1δD. rerio0.033 µM [0.023-0.048]Ach (100 uM)Rybin et al. (2020)
α1β1δεD. rerio0.058 µM[0.04-0.085]Ach (10 uM)Rybin et al. (2020)
H. sapiens4.1 µM[2.9-5.9]Ach (10 uM)Rybin et al. (2020)
M. musculus3.6 µM[2.6-4.9]Ach (10 uM)Rybin et al. (2020)
α1β1εD. rerio0.034 µM[0.022-0.053]Ach (10 uM)Rybin et al. (2020)
α1β1γδD. rerio0.061 µM[0.049-0.076]Ach (10 uM)Rybin et al. (2020)
H. sapiens19.6 µM[13.8-27.7]Ach (10 uM)Rybin et al. (2020)
M. musculus44.4 µM[25.4-77.5]Ach (10 uM)Rybin et al. (2020)
α3β4R. norvegicus>>10^8 µMAch (10 uM)Rybin et al. (2020)
α4β2R. norvegicus>>10^8 µMAch (10 uM)Rybin et al. (2020)
α9α10H. sapiens>>10^8 µMAch (10 uM)Rybin et al. (2020)

References
Rybin, M.J., O’Brien, H., Ramiro, I.B.L., Azam, L., McIntosh, J.M., Olivera, B.M., Safavi-Hemami, H. and Yoshikami, D. (2020) αM-Conotoxin MIIIJ Blocks Nicotinic Acetylcholine Receptors at Neuromuscular Junctions of Frog and Fish Toxins (Basel) 12

Internal links
Nucleic acids
Structure

External links

Tools