MilIA (P09015) Protein Card

General Information
Name MilIA
Organism Conus milneedwardsi
Organism region South Africa,Indo-Pacific
Organism diet piscivorous
Protein Type Wild type

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
DMCCHPACMNHFNC(nh2)
Modified residues
positionsymbolname
15nh2C-term amidation
Sequence evidence protein level
Average Mass 1620.88
Monoisotopic Mass 1619.50
Isoelectric Point 6.81
Extinction Coefficient [280nm] NA

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α1β1δεH. sapiens11180 nM+/-788911.2ACh(200 µM)Peigneur,S. et al. (2019)
α1β1γδH. sapiens13130 nM+/-11250.7ACh(200 µM)Peigneur,S. et al. (2019)
α2β4H. sapiens>10 uMACh(200 µM)Peigneur,S. et al. (2019)
α4β2H. sapiens>10 uMACh(200 µM)Peigneur,S. et al. (2019)
α4β4H. sapiens>10 uMACh(200 µM)Peigneur,S. et al. (2019)
α7H. sapiens>10 uMACh(100 µM)Peigneur,S. et al. (2019)
α9α10H. sapiens>10 uMACh(500 µM)Peigneur,S. et al. (2019)

Synthetic variants
MilIA[M9G,N10K]DMCCHPACGKHFNC(nh2)
MilIA[M9G]DMCCHPACGNHFNC(nh2)
MilIA[N10K]DMCCHPACMKHFNC(nh2)
MilIA[del1,M2R,M9G,N10K,H11K]RCCHPACGKKFNC(nh2)
MilIA[del1,M2R]RCCHPACMNHFNC(nh2)

References
Peigneur,S., Devi,P., Seldeslachts,A., Ravichandran,S., Quinton,L. and Tytgat,J. (2019) Structure-Function Elucidation of a New α-Conotoxin, MilIA, from Conus milneedwardsi Mar Drugs 17:535

Internal links
Nucleic acids
Structure

External links

Tools