GID (P00098) Protein Card

General Information
Name GID
Alternative name(s) G1d
Organism Conus geographus (Geography cone)
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor GID precursor (5711)
GID precursor (6874)

Classification
Conopeptide class conotoxin
Gene superfamily A superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
IRD(Gla)CCSNPACRVNNOHVC
Modified residues
positionsymbolname
4GlaGamma carboxylic glutamic acid
16O4-Hydroxyproline
Sequence evidence protein level
Average Mass 2186.39
Monoisotopic Mass 2184.85
Isoelectric Point 8.31
Extinction Coefficient [280nm] NA

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α3β2H. sapiens0.01 uMAch (1 uM)Leffler,A.E. et al. (2017)
R. norvegicus3.4nM+/-0.51.3100uM AchMillard EL et al. (2009)
3.1nM1.16100uM AchNicke,A. et al. (2003)
Unknown5.1nM1.6100uM AchDutertre,S. et al. (2005)
α3β4H. sapiens>10 uMAch (5 uM)Leffler,A.E. et al. (2017)
R. norvegicus>5uM100uM AchNicke,A. et al. (2003)
α4β2H. sapiens(ToxDock prediction)3 uMAch (0.5 uM)Leffler,A.E. et al. (2017)
R. norvegicus152nM1.13100uM AchNicke,A. et al. (2003)
128.6nM+/-13.11.2100uM AchMillard EL et al. (2009)
Unknown390nM1.3100uM AchDutertre,S. et al. (2005)
α4β4R. norvegicus>5uM100uM AchNicke,A. et al. (2003)
α7H. sapiens0.1 uMAch (30 uM)Leffler,A.E. et al. (2017)
R. norvegicus4.5nM1.06100uM AchNicke,A. et al. (2003)
5.1nM+/-1.31.3100uM Ach or nicotineMillard EL et al. (2009)

Synthetic variants
GID [(Gla)4A]IRDACCSNPACRVNNOHVC
GID [(Gla)4E, A10Q]IRDECCSNPQCRVNNOHVC
GID [(Gla)4E, A10S]IRDECCSNPSCRVNNOHVC
GID [(Gla)4E, A10T]IRDECCSNPTCRVNNOHVC
GID [(Gla)4E, A10V]IRDECCSNPVCRVNNOHVC
GID [(Gla)4E, N15H] IRDECCSNPACRVNHOHVC
GID [(Gla)4E, N15K]IRDECCSNPACRVNKOHVC
GID [(Gla)4E, V13F]IRDECCSNPACRFNNOHVC
GID [(Gla)4E, V13I]IRDECCSNPACRINNOHVC
GID [(Gla)4E, V13L]IRDECCSNPACRLNNOHVC
GID [(Gla)4E, V13S]IRDECCSNPACRSNNOHVC
GID [(Gla)4E, V13T]IRDECCSNPACRTNNOHVC
GID [(Gla)4E, V13W]IRDECCSNPACRWNNOHVC
GID [(Gla)4E, V13Y]IRDECCSNPACRYNNOHVC
GID [(Gla)4E, V18N]IRDECCSNPACRVNNOHNC
GID [(Gla)4E, V18Q]IRDECCSNPACRVNNOHQC
GID [(Gla)4E, V18Y]IRDECCSNPACRVNNOHYC
GID [(Gla)4E]IRDECCSNPACRVNNOHVC
GID [(Gla)4Q]IRDQCCSNPACRVNNOHVC
GID [D3A,(Gla)4E]IRAECCSNPACRVNNOHVC
GID [D3N, (Gla)4E]IRNECCSNPACRVNNOHVC
GID [D3N,E4Q]IRNQCCSNPACRVNNOHVC
GID [I1A, (Gla)4E]ARDECCSNPACRVNNOHVC
GID [R12A]IRD(Gla)CCSNPACAVNNOHVC
GID [R2A, (Gla)4E]IADECCSNPACRVNNOHVC
GID [R2K, (Gla)4E]IKDECCSNPACRVNNOHVC
GID amidated [(GLA)4E]IRDECCSNPACRVNNOHVC(nh2)
GID[(GLA)4E, H17A]IRDECCSNPACRVNNOAVC
GID[(GLA)4E, N14A]IRDECCSNPACRVANOHVC
GID[(GLA)4E, N15A]IRDECCSNPACRVNAOHVC
GID[(GLA)4E, N8A]IRDECCSAPACRVNNOHVC
GID[(GLA)4E, O16A]IRDECCSNPACRVNNAHVC
GID[(GLA)4E, O16P]IRDECCSNPACRVNNPHVC
GID[(GLA)4E, P9A]IRDECCSNAACRVNNOHVC
GID[(GLA)4E, S7A]IRDECCANPACRVNNOHVC
GID[(GLA)4E, V13A]IRDECCSNPACRANNOHVC
GID[(GLA)4E, V18A]IRDECCSNPACRVNNOHAC
GID[Del1-2,D3A, (GLA)4E]AECCSNPACRVNNOHVC
GID[Del1-3, (GLA)4A]ACCSNPACRVNNOHVC
GID[Del1-3, (GLA)4E]ECCSNPACRVNNOHVC
GID[Del4]CCSNPACRVNNOHVC
GID[R12A]IRD(Gla)CCSNPACRVNNOHVC
GID[del1, R2A, (GLA)4E]ADECCSNPACRVNNOHVC

References
Nicke,A., Loughnan,M.L., Millard,E.L., Alewood,P.F., Adams,D.J., Daly,N.L., Craik,D.J. and Lewis,R.J. (2003) Isolation, Structure, and Activity of GID, a Novel alpha 4/7-Conotoxin with an Extended N-terminal Sequence J. Biol. Chem. 278:3137-3144
Millard EL, Nevin ST, Loughnan ML, Nicke A, Clark RJ, Alewood PF, Lewis RJ, Adams DJ, Craik DJ, Daly NL (2009) Inhibition of neuronal nicotinic acetylcholine receptor subtypes by alpha-Conotoxin GID and analogues. J Biol Chem. 284:4944-4951
Dutertre,S., Nicke,A., and Lewis,R.J. (2005) Beta2 subunit contribution to 4/7 alpha-conotoxin binding to the nicotinic acetylcholine receptor. J. Biol. Chem. 280:30460-30468
Leffler,A.E., Kuryatov,A., Zebroski,H.A., Powell,S.R., Filipenko,P., Hussein,A.K., Gorson,J., Heizmann,A., Lyskov,S., Tsien,R.W., Poget,S.F., Nicke,A., Lindstrom,J., Rudy,B., Bonneau,R. and Holford,M. (2017) Discovery of peptide ligands through docking and virtual screening at nicotinic acetylcholine receptor homology models. Proc. Natl. Acad. Sci. U.S.A. 114

Internal links
Protein Precursor GID precursor (5711)
GID precursor (6874)
Nucleic acids
Structure THREE-DIMENSIONAL SOLUTION STRUCTURE OF ALPHA-CONOTOXIN GID BY NMR SPECTROSCOPY

External links
UniProtKB/Swiss-Prot P60274
Ncbi 1MTQ_A, P60274

Tools