GID (P00098) Protein Card

General Information
Name GID
Alternative name(s) G1d
Organism Conus geographus (Geography cone)
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Protein precursor GID precursor (5711)
GID precursor (6874)

Classification
Conopeptide class conotoxin
Gene superfamily A superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
IRD(Gla)CCSNPACRVNNOHVC
Modified residues
positionsymbolname
4GlaGamma carboxylic glutamic acid
16O4-Hydroxyproline
Sequence evidence protein level
Average Mass 2186.39
Monoisotopic Mass 2184.85
Isoelectric Point 8.31
Extinction Coefficient [280nm] NA

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α3β2R. norvegicus3.1nM1.16100uM AchNicke,A. et al. (2003)
3.4nM+/-0.51.3100uM AchMillard EL et al. (2009)
Unknown5.1nM1.6100uM AchDutertre,S. et al. (2005)
α3β4R. norvegicus>5uM100uM AchNicke,A. et al. (2003)
α4β2R. norvegicus152nM1.13100uM AchNicke,A. et al. (2003)
128.6nM+/-13.11.2100uM AchMillard EL et al. (2009)
Unknown390nM1.3100uM AchDutertre,S. et al. (2005)
α4β4R. norvegicus>5uM100uM AchNicke,A. et al. (2003)
α7R. norvegicus5.1nM+/-1.31.3100uM Ach or nicotineMillard EL et al. (2009)
4.5nM1.06100uM AchNicke,A. et al. (2003)

Synthetic variants
GID [R12A]CVHONNVACAPNSCC(Gla)DRI
GID*CVHONNVRCAPNSCCEDRI
GID*-NH2(nh2)CVHONNVRCAPNSCCEDRI
GID*[A10S]CVHONNVRCSPNSCCEDRI
GID*[A10T]CVHONNVRCTPNSCCEDRI
GID*[A10V]CVHONNVRCVPNSCCEDRI
GID*[D3A]CVHONNVRCAPNSCCEARI
GID*[D3N,E4Q]CVHONNVRCAPNSCCQNRI
GID*[D3N]CVHONNVRCAPNSCCENRI
GID*[Del1,R2A]CVHONNVRCAPNSCCEDA
GID*[Del1-2,D3A]CVHONNVRCAPNSCCEA
GID*[Del1-3]CVHONNVRCAPNSCCE
GID*[E4A]CVHONNVRCAPNSCCADRI
GID*[E4Q]CVHONNVRCAPNSCCQDRI
GID*[H17A]CVAONNVRCAPNSCCEDRI
GID*[I1A]CVHONNVRCAPNSCCEDRA
GID*[N14A]CVHONAVRCAPNSCCEDRI
GID*[N15A]CVHOANVRCAPNSCCEDRI
GID*[N15H]CVHOHNVRCAPNSCCEDRI
GID*[N15K]CVHOKNVRCAPNSCCEDRI
GID*[N8A]CVHONNVRCAPASCCEDRI
GID*[O16A]CVHANNVRCAPNSCCEDRI
GID*[O16P]CVHPNNVRCAPNSCCEDRI
GID*[P9A]CVHONNVRCAANSCCEDRI
GID*[R12A]CVHONNVACAPNSCCEDRI
GID*[R2A]CVHONNVRCAPNSCCEDAI
GID*[R2K]CVHONNVRCAPNSCCEDKI
GID*[S7A]CVHONNVRCAPNACCEDRI
GID*[V13A]CVHONNARCAPNSCCEDRI
GID*[V13F]CVHONNFRCAPNSCCEDRI
GID*[V13I]CVHONNIRCAPNSCCEDRI
GID*[V13L]CVHONNLRCAPNSCCEDRI
GID*[V13S]CVHONNSRCAPNSCCEDRI
GID*[V13T]CVHONNTRCAPNSCCEDRI
GID*[V13W]CVHONNWRCAPNSCCEDRI
GID*[V13Y]CVHONNYRCAPNSCCEDRI
GID*[V18A]CAHONNVRCAPNSCCEDRI
GID*[V18N]CNHONNVRCAPNSCCEDRI
GID*[V18Q]CQHONNVRCAPNSCCEDRI
GID*[V18Y]CYHONNVRCAPNSCCEDRI
GID[Del1-3,Gla4A]CVHONNVRCAPNSCCA
GID[Del4]CVHONNVRCAPNSCC
GID[R12A]CVHONNVRCAPNSCC(Gla)DRI

References
Nicke,A., Loughnan,M.L., Millard,E.L., Alewood,P.F., Adams,D.J., Daly,N.L., Craik,D.J. and Lewis,R.J. (2003) Isolation, Structure, and Activity of GID, a Novel alpha 4/7-Conotoxin with an Extended N-terminal Sequence J. Biol. Chem. 278:3137-3144
Millard EL, Nevin ST, Loughnan ML, Nicke A, Clark RJ, Alewood PF, Lewis RJ, Adams DJ, Craik DJ, Daly NL (2009) Inhibition of neuronal nicotinic acetylcholine receptor subtypes by alpha-Conotoxin GID and analogues. J Biol Chem. 284:4944-4951
Dutertre,S., Nicke,A., and Lewis,R.J. (2005) Beta2 subunit contribution to 4/7 alpha-conotoxin binding to the nicotinic acetylcholine receptor. J. Biol. Chem. 280:30460-30468

Internal links
Protein Precursor GID precursor (5711)
GID precursor (6874)
Nucleic acids
Structure THREE-DIMENSIONAL SOLUTION STRUCTURE OF ALPHA-CONOTOXIN GID BY NMR SPECTROSCOPY

External links
UniProtKB/Swiss-Prot P60274
Ncbi P60274, 1MTQ_A

Tools