PnIB (P00099) Protein Card

General Information
Name PnIB
Alternative name(s) PnMGMR-01,Pn1b
Organism Conus pennaceus
Organism region Indo-Pacific
Organism diet molluscivorous
Protein Type Wild type
Protein precursor PnIB precursor (36)
Notes The presence of a sulfotyrosine was discovered in 1999, while PnIB was isolated from the venom in 1994. There exists some confusion in the literature regarding the use of PnIB with sulfotyrosine or of a synthetic version without. See the card of PnIB [sTy15Y] for the activity data.

Classification
Conopeptide class conotoxin
Gene superfamily A superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
GCCSLPPCALSNPD(sTy)C(nh2)
Modified residues
positionsymbolname
15sTySulfotyrosine
17nh2C-term amidation
Sequence evidence protein level
Average Mass 1716.92
Monoisotopic Mass 1715.56
Isoelectric Point 2.19
Extinction Coefficient [280nm] 1490.00

Synthetic variants
PnIB [sTy15Y](nh2)CYDPNSLACPPLSCCG

References
Fainzilber,M., Hasson,A., Oren,R., Burlingame,A.L., Gordon,D., Spira,M.E. and Zlotkin,E. (1994) New mollusc-specific alpha-conotoxins block Aplysia neuronal acetylcholine receptors Biochemistry 33:9523-9529
Wolfender,J.L., Chu,F., Ball,H., Wolfender,F., Fainzilber,M., Baldwin,M.A. and Burlingame,A.L. (1999) Identification of tyrosine sulfation in Conus pennaceus conotoxins alpha-PnIA and alpha-PnIB: further investigation of labile sulfo- and phosphopeptides by electrospray, matrix-assisted laser desorption/ionization (MALDI) and atmospheric pressure MALDI mass spectrometry J Mass Spectrom 34:447-454

Internal links
Protein Precursor PnIB precursor (36)
Nucleic acids
Structure ALPHA-CONOTOXIN PNIB FROM CONUS PENNACEUS

External links
Ncbi 1AKG_A, AAB31732

Tools