Conantokin-T (P01269) Protein Card

General Information
Name Conantokin-T
Alternative name(s) Con-T
Organism Conus tulipa (tulip cone)
Organism region Indo-Pacific
Organism diet piscivorous
Protein Type Wild type
Notes

sleeping in young mice; hyperactivity in older mice (NMDA receptor)

Dutt et al., 2019 reported that conantokin-T (1 μM) showed no effect on the zebrafish larvae.

Classification
Conopeptide class conantokin
Gene superfamily
Pharmacological family

Sequence
GE(Gla)(Gla)YQKML(Gla)NLR(Gla)AEVKKNA(nh2)
Modified residues
positionsymbolname
3GlaGamma carboxylic glutamic acid
4GlaGamma carboxylic glutamic acid
10GlaGamma carboxylic glutamic acid
14GlaGamma carboxylic glutamic acid
22nh2C-term amidation
Sequence evidence protein level
Average Mass 2683.84
Monoisotopic Mass 2682.21
Isoelectric Point 4.51
Extinction Coefficient [280nm] 1490.00

References
Malmberg,A.B., Gilbert,H., McCabe,R.T. and Basbaum,A.I. (2003) Powerful antinociceptive effects of the cone snail venom-derived subtype-selective NMDA receptor antagonists conantokins G and T Pain 101:109-116
Haack,J.A., Rivier,J., Parks,T.N., Mena,E.E., Cruz,L.J. and Olivera,B.M. (1990) Conantokin-T. A gamma-carboxyglutamate containing peptide with N-methyl-d-aspartate antagonist activity J. Biol. Chem. 265:6025-6029
Olivera,B.M., Rivier,J., Clark,C., Ramilo,C.A., Corpuz,G.P., Abogadie,F.C., Mena,E.E., Woodward,S.R., Hillyard,D.R. and Cruz,L.J. (1990) Diversity of Conus neuropeptides Science 249:257-263
Klein,R.C., Prorok,M., Galdzicki,Z. and Castellino,F.J. (2001) The amino acid residue at sequence position 5 in the conantokin peptides partially governs subunit-selective antagonism of recombinant N-methyl-D-aspartate receptors J. Biol. Chem. 276:26860-26867
Warder,S.E., Chen,Z., Zhu,Y., Prorok,M., Castellino,F.J. and Ni,F. (1997) The NMR solution structure of the NMDA receptor antagonist, conantokin-T, in the absence of divalent metal ions FEBS Lett. 411:19-26
Skjaerbaek,N., Nielsen,K.J., Lewis,R.J., Alewood,P. and Craik,D.J. (1997) Determination of the solution structures of conantokin-G and conantokin-T by CD and NMR spectroscopy J. Biol. Chem. 272:2291-2299
Balsara,R., Li,N., Weber-Adrian,D., Huang,L. and Castellino,F.J. (2012) Opposing action of conantokin-G on synaptically and extrasynaptically-activated NMDA receptors. Neuropharmacology 62:2227-2238
Dutt,M., Giacomotto,J., Ragnarsson,L., Andersson,Å., Brust,A., Dekan,Z., Alewood,P.F., and Lewis,R.J. (2019) The α1-adrenoceptor inhibitor ρ-TIA facilitates net hunting in piscivorous Conus tulipa Scientific reports 9:17841

Internal links
Nucleic acids
Structure Nmda Receptor Antagonist, Conantokin-T, Nmr, 17 Structures

External links
UniProtKB/Swiss-Prot P17684
Ncbi 1NOT_A, 1ONT_A, P17684

Tools