OIVA (P01397) Protein Card

General Information

Name	OIVA
Alternative name(s)	O4a
Organism	Conus obscurus
Organism region	Indo-Pacific
Organism diet	piscivorous
Protein Type	Wild type
Notes	The superfamily assignment is not supported by any available data but the cDNA extraction method is based on the conservation of 5' and 3' UTR.

Classification

Conopeptide class	conotoxin
Gene superfamily	A superfamily
Cysteine framework	IV
Pharmacological family	alpha conotoxin

Sequence

CCGVONAACHOCVCKNTC(nh2)
Modified residues	position symbol name 5 O 4-Hydroxyproline 11 O 4-Hydroxyproline 19 nh2 C-term amidation
Sequence evidence	protein level
Average Mass	1848.14
Monoisotopic Mass	1846.65
Isoelectric Point	10.95
Extinction Coefficient [280nm]	NA

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism	IC50		Agonist	Ref
α1β1δε	M. musculus	310nM	+/-80	1-10uM Ach	Teichert,R.W. et al. (2006)
α1β1γδ	M. musculus	510pM	+/-130	1-10uM Ach	Teichert,R.W. et al. (2006)

Percentage inhibition: 5-HT₃ receptors

Target	Organism	% inhibition		Concentration	Agonist	Ref
5-HT3A	H. sapiens	90	+/-10	10uM	3uM serotonin	Teichert,R.W. et al. (2006)

Percentage inhibition: Nicotinic acetylcholine receptors

Target	Organism	% inhibition		Concentration	Agonist	Ref
α1β1δε	H. sapiens	100		10uM	1-10uM Ach	Teichert,R.W. et al. (2006)
	M. musculus	90		10uM	1-10uM Ach	Teichert,R.W. et al. (2006)
α1β1γδ	H. sapiens	100		10uM	1-10uM Ach	Teichert,R.W. et al. (2006)
	M. musculus	100		10uM	1-10uM Ach	Teichert,R.W. et al. (2006)
α2β4	H. sapiens	0		10uM	100uM Ach	Teichert,R.W. et al. (2006)
α3β4	H. sapiens	0		10uM	100uM Ach	Teichert,R.W. et al. (2006)
α4β2	H. sapiens	5	+/-5	10uM	100uM Ach	Teichert,R.W. et al. (2006)
	M. musculus	10	+/-10	10uM	100uM Ach	Teichert,R.W. et al. (2006)
α6/α3β2β3	R. norvegicus	0		10uM	100uM Ach	Teichert,R.W. et al. (2006)
α7	H. sapiens	0		10uM	200uM Ach	Teichert,R.W. et al. (2006)

Synthetic variants

OIVA [H10P]	CCGVONAACPOCVCKNTC(nh2)
OIVA [K15N]	CCGVONAACHOCVCNNTC(nh2)

References

Teichert,R.W., Rivier,J., Dykert,J., Cervini,L., Gulyas,J., Bulaj,G., Ellison,M. and Olivera,B.M. (2004) AlphaA-Conotoxin OIVA defines a new alphaA-conotoxin subfamily of nicotinic acetylcholine receptor inhibitors Toxicon 44:207-214

Teichert,R.W., Lopez-Vera,E., Gulyas,J., Watkins,M., Rivier,J. and Olivera,B.M. (2006) Definition and characterization of the short alphaA-conotoxins: a single residue determines dissociation kinetics from the fetal muscle nicotinic acetylcholine receptor Biochemistry 45:1304-1312

Internal links

Nucleic acids
Structure

External links

UniProtKB/Swiss-Prot	P69746
Ncbi	P69746

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Tuesday 5 December 2023