TxVIA (P01552) Protein Card

General Information
Name TxVIA
Alternative name(s) KK-0,King-Kong 0,Tx6a,TxIA
Organism Conus textile (Cloth-of-gold cone)
Organism region Indo-Pacific
Organism diet molluscivorous
Protein Type Wild type
Protein precursor TxVIA precursor (598)
TxVIA precursor (1094)
TxVIA precursor (3025)
TxVIA precursor (3026)
Notes
  • TxVIA makes lobsters assume dominant posture; contraction in snails; no symptom in mice. It was also recently reported to have insecticidal activity, including for the cabbage moth and house fly (Bruce et al. 2011). It has no effect on grey field slug Deroceras reticulatum (Bruce et al. 2011).
  • The methionine was found to be oxidated by Dobson 2012.
  • TxVIA has no effect on mammalian Nav1.2 and Nav1.7 even at high concentrations (10 µM)(Wang et al., 2021).
  • TxVIA activates Cav3.1 (Wang et al., 2021).

    • Classification
    Conopeptide class conotoxin
    Gene superfamily O1 superfamily
    Cysteine framework VI/VII
    Pharmacological family delta conotoxin

    Sequence
    WCKQSGEMCNLLDQNCCDGYCIVLVCT
    Sequence evidence protein level
    Average Mass 3036.49
    Monoisotopic Mass 3034.19
    Isoelectric Point 4.79
    Extinction Coefficient [280nm] 6990.00

    Activity

    IC50: Voltage-Gated Calcium Channels

    TargetOrganismIC50nhillRef
    Cav3.2H. sapiens0.24 uM+/-0.110.11Wang D et al. (2020)

    References
    Hillyard,D.R., Olivera,B.M., Woodward,S., Corpuz,G.P., Gray,W.R., Ramilo,C.A. and Cruz,L.J. (1989) A molluscivorous Conus toxin: conserved frameworks in conotoxins Biochemistry 28:358-361
    Fainzilber,M., Gordon,D., Hasson,A., Spira,M.E. and Zlotkin,E. (1991) Mollusc-specific toxins from the venom of Conus textile neovicarius Eur. J. Biochem. 202:589-595
    Kohno,T., Sasaki,T., Kobayashi,K., Fainzilber,M. and Sato,K. (2002) Three-dimensional solution structure of the sodium channel agonist/antagonist delta-conotoxin TxVIA J. Biol. Chem. 277:36387-36391
    Ueberheide,B.M., Fenyö,D., Alewood,P.F. and Chait,B.T. (2009) Rapid sensitive analysis of cysteine rich peptide venom components. Proc. Natl. Acad. Sci. U.S.A. 106:6910-6915
    Bruce,C., Fitches,E.C., Chougule,N., Bell,H.A. and Gatehouse,J.A. (2011) Recombinant conotoxin, TxVIA, produced in yeast has insecticidal activity. Toxicon 58:93-100
    Bhatia,S., Kil,Y.J., Ueberheide,B., Chait,B.T., Tayo,L., Cruz,L., Lu,B., Yates,J.R. 3rd. and Bern,M. (2012) Constrained de novo sequencing of conotoxins. J. Proteome Res. 11:4191-4200
    Dobson,R., Collodoro,M., Gilles,N., Turtoi,A., De Pauw,E. and Quinton,L. (2012) Secretion and maturation of conotoxins in the venom ducts of Conus textile. Toxicon 60:1370-1379
    Wang D, Himaya SWA, Giacomotto J, Hasan MM, Cardoso FC, Ragnarsson L, Lewis RJ. (2020) Characterisation of δ-Conotoxin TxVIA as a Mammalian T-Type Calcium Channel Modulator Mar Drugs 18:343

    Internal links
    Protein Precursor TxVIA precursor (598)
    TxVIA precursor (1094)
    TxVIA precursor (3025)
    TxVIA precursor (3026)
    Nucleic acids
    Structure THREE-DIMENSIONAL STRUCTURE IN SOLUTION OF THE SODIUM CHANNEL AGONIST/ANTAGONIST DELTA-CONOTOXIN TXVIA

    External links
    Ncbi 1FU3_A, AAB20785

    Tools