GIIIB (P01566) Protein Card

General Information

Name	GIIIB
Alternative name(s)	GTx-II,Geographutoxin II,Myotoxin II
Organism	Conus geographus (Geography cone)
Organism region	Indo-Pacific
Organism diet	piscivorous
Protein Type	Wild type
Protein precursor	GIIIB precursor (5726)
Notes	Bind to tetrodotoxin sensitive Na channel (Cruz et al 1985) and, presumably, interact with site 1 in the Na channel pore.

Classification

Conopeptide class	conotoxin
Gene superfamily	M superfamily
Cysteine framework	III
Pharmacological family	mu conotoxin

Sequence

RDCCTOORKCKDRRCKOMKCCA(nh2)
Modified residues	position symbol name 6 O 4-Hydroxyproline 7 O 4-Hydroxyproline 17 O 4-Hydroxyproline 23 nh2 C-term amidation
Sequence evidence	protein level
Average Mass	2640.15
Monoisotopic Mass	2638.14
Isoelectric Point	12.18
Extinction Coefficient [280nm]	NA

References

Cruz,L.J., Gray,W.R., Olivera,B.M., Zeikus,R.D., Kerr,L., Yoshikami,D. and Moczydlowski,E. (1985) Conus geographus toxins that discriminate between neuronal and muscle sodium channels J. Biol. Chem. 260:9280-9288

Sato,S., Nakamura,H., Ohizumi,Y., Kobayashi,J. and Hirata,Y. (1983) The amino acid sequences of homologous hydroxyproline-containing myotoxins from the marine snail Conus geographus venom FEBS Lett. 155:277-280

Ott,K.H., Becker,S., Gordon,R.D. and Ruterjans,H. (1991) Solution structure of mu-conotoxin GIIIA analysed by 2D-NMR and distance geometry calculations FEBS Lett. 278:160-166

Lancelin,J.M., Kohda,D., Tate,S., Yanagawa,Y., Abe,T., Satake,M. and Inagaki,F. (1991) Tertiary structure of conotoxin GIIIA in aqueous solution Biochemistry 30:6908-6916

Wakamatsu,K., Kohda,D., Hatanaka,H., Lancelin,J.M., Ishida,Y., Oya,M., Nakamura,H., Inagaki,F. and Sato,K. (1992) Structure-activity relationships of mu-conotoxin GIIIA: structure determination of active and inactive sodium channel blocker peptides by NMR and simulated annealing calculations Biochemistry 31:12577-12584

Hill,J.M., Alewood,P.F. and Craik,D.J. (1996) Three-dimensional solution structure of mu-conotoxin GIIIB, a specific blocker of skeletal muscle sodium channels Biochemistry 35:8824-8835

Internal links

Protein Precursor	GIIIB precursor (5726)
Nucleic acids
Structure	MU-CONOTOXIN GIIIB, NMR

External links

UniProtKB/Swiss-Prot	P01524
Ncbi	1GIB_A, P01524

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Sunday 2 April 2023