EIVA (P01635) Protein Card

General Information

Name	EIVA
Alternative name(s)	E4a
Organism	Conus ermineus (Atlantic fish-hunting cone)
Organism region	Eastern Atlantic and Mediterranean,Western Atlantic and Caribbean
Organism diet	piscivorous
Protein Type	Wild type

Classification

Conopeptide class	conotoxin
Gene superfamily
Cysteine framework	IV
Pharmacological family	alpha conotoxin

Sequence

GCCGPYONAACHOCGCKVGROOYCDROSGG(nh2)
Modified residues	position symbol name 7 O 4-Hydroxyproline 13 O 4-Hydroxyproline 21 O 4-Hydroxyproline 22 O 4-Hydroxyproline 27 O 4-Hydroxyproline 31 nh2 C-term amidation
Sequence evidence	protein level
Average Mass	3096.42
Monoisotopic Mass	3094.18
Isoelectric Point	10.05
Extinction Coefficient [280nm]	2980.00

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism	IC50		Agonist	Ref
α1β1δ	M. musculus	15nM		10uM Ach	Jacobsen,R.B. et al. (1997)
α1β1γδ	T. marmorata	17nM		10uM Ach	Jacobsen,R.B. et al. (1997)
α1β1;γ	M. musculus	11nM		10uM Ach	Jacobsen,R.B. et al. (1997)

References

Jacobsen,R.B., Yoshikami,D., Ellison,M., Martinez,J., Gray,W.R., Cartier,G.E., Shon,K.-J., Groebe,D.R., Abramson,S.N., Olivera,B.M. and McIntosh,J.M. (1997) Differential targeting of nicotinic acetylcholine receptors by novel alphaA-conotoxins J. Biol. Chem. 272:22531-22537

Chi,S.W., Park,K.H., Suk,J.E., Olivera,B.M., McIntosh,J.M. and Han,K.H. (2003) Solution conformation of alphaA-conotoxin EIVA, a potent neuromuscular nicotinic acetylcholine receptor antagonist from Conus ermineus J. Biol. Chem. 278:42208-42213

Internal links

Nucleic acids
Structure	SOLUTION CONFORMATION OF ALPHAA-CONOTOXIN EIVA

External links

UniProtKB/Swiss-Prot	P58782
Ncbi	1PQR_A, P58782

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Tuesday 21 March 2023