CVID (P02549) Protein Card

General Information

Name	CVID
Alternative name(s)	AM-336,AM336,Ca061,leconotide
Organism	Conus catus
Organism region	Indo-Pacific
Organism diet	piscivorous
Protein Type	Wild type
Protein precursor	CVID precursor (1569) CVID precursor (2872)

Classification

Conopeptide class	conotoxin
Gene superfamily	O1 superfamily
Cysteine framework	VI/VII
Pharmacological family	omega conotoxin

Sequence

CKSKGAKCSKLMYDCCSGSCSGTVGRC(nh2)

Modified residues

position	symbol	name
28	nh2	C-term amidation

Sequence evidence

protein level

Average Mass

2756.22

Monoisotopic Mass

2754.13

Isoelectric Point

11.59

Extinction Coefficient [280nm]

1490.00

Activity

Ki: Voltage-Gated Calcium Channels

Target	Organism	Ki	Competitor	Ref
Ca_v2.1	R. norvegicus	55000 (49000-62000) nM	¹²⁵I-MVIIC	Lewis,R.J. et al. (2000)
Ca_v2.2	R. norvegicus	0.04 nM	¹²⁵I-GVIA	Mould,J. et al. (2004)
		0.070 (0.058-0.077) nM	¹²⁵I-GVIA	Lewis,R.J. et al. (2000)

Synthetic variants

CVID[K10R]	CKSKGAKCSRLMYDCCSGSCSGTVGRC(nh2)
CVID[K2A,K10R]	CASKGAKCSRLMYDCCSGSCSGTVGRC(nh2)

References

Lewis,R.J., Nielsen,K.J., Craik,D.J., Loughnan,M.L., Adams,D.A., Sharpe,I.A., Luchian,T., Adams,D.J., Bond,T., Thomas,L., Jones,A., Matheson,J.L., Drinkwater,R., Andrews,P.R. and Alewood,P.F. (2000) Novel omega-conotoxins from Conus catus discriminate among neuronal calcium channel subtypes J. Biol. Chem. 275:35335-35344

Adams,D.J., Smith,A.B., Schroeder,C.I., Yasuda,T. and Lewis,R.J. (2003) Omega-conotoxin CVID inhibits a pharmacologically distinct voltage-sensitive calcium channel associated with transmitter release from preganglionic nerve terminals J. Biol. Chem. 278:4057-4062

Mould,J., Yasuda,T., Schroeder,C.I., Beedle,A.M., Doering,C.J., Zamponi,G.W., Adams,D.J. and Lewis,R.J. (2004) The alpha2delta auxiliary subunit reduces affinity of omega-conotoxins for recombinant N-type (Cav2.2) calcium channels. J. Biol. Chem. 279:34705-34714

Himaya, S.W.A., Jin, A.H., Dutertre, S., Giacomotto, J., Mohialdeen, H., Vetter, I., Alewood, P.F. and Lewis, R.J. (2015) Comparative venomics reveals the complex prey capture strategy of the piscivorous cone snail Conus catus. Journal of proteome research 14:4372-4381

Internal links

Protein Precursor	CVID precursor (1569) CVID precursor (2872)
Nucleic acids
Structure	NMR structure of CVID

External links

Ncbi

P58920

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Tuesday 28 November 2023