PrXA (P02859) Protein Card

General Information

Name	PrXA
Alternative name(s)	alphaC-PrXA
Organism	Conus parius
Organism region	Indo-Pacific
Organism diet	piscivorous
Protein Type	Wild type
Protein precursor	PrXA precursor (4224)
Notes	El-Aziz et al., 2017 reported that DNA aptamers can counteract PrXA activity, neutralizing the toxin-induced displacement of [125I]-α-bungarotoxin onto nicotinic receptors, its inhibition of diaphragm muscle contraction, as well as its lethality to mice.

Classification

Conopeptide class	conotoxin
Gene superfamily	C superfamily
Pharmacological family	alpha conotoxin

Sequence

TYGIYDAKPOFSCAGLRGGCVLPONLROKFKE(nh2)
Modified residues	position symbol name 10 O 4-Hydroxyproline 24 O 4-Hydroxyproline 28 O 4-Hydroxyproline 33 nh2 C-term amidation
Sequence evidence	protein level
Average Mass	3541.11
Monoisotopic Mass	3538.78
Isoelectric Point	11.38
Extinction Coefficient [280nm]	2980.00

Activity

IC50: Voltage-Gated Sodium Channels

Target	Organism		IC50		Agonist	Ref
Nav1.4	M. musculus		>>10 μM		ACh (100 μM)	Jimenez,E.C. and Olivera,B.M. and Teichert,R.W. (2007)
Nav1.6	M. musculus		>>10 μM		ACh (100 μM)	Jimenez,E.C. and Olivera,B.M. and Teichert,R.W. (2007)

IC50: Nicotinic acetylcholine receptors

Target	Organism		IC50		Agonist	Ref
α1β1δε	M. musculus	(adult muscle)	1.8 nM	[1.5-2.2]	ACh (100 μM)	Jimenez,E.C. and Olivera,B.M. and Teichert,R.W. (2007)
α1β1γδ	M. musculus	(fetal muscle)	3.3 nM	[2.8-3.7]	ACh (100 μM)	Jimenez,E.C. and Olivera,B.M. and Teichert,R.W. (2007)
α2β4	R. norvegicus		>>10 μM		ACh (100 μM)	Jimenez,E.C. and Olivera,B.M. and Teichert,R.W. (2007)
α3β2	R. norvegicus		>>10 μM		ACh (100 μM)	Jimenez,E.C. and Olivera,B.M. and Teichert,R.W. (2007)
α3β4	R. norvegicus		>>10 μM		ACh (100 μM)	Jimenez,E.C. and Olivera,B.M. and Teichert,R.W. (2007)
α4β2	H. sapiens		>>10 μM		ACh (100 μM)	Jimenez,E.C. and Olivera,B.M. and Teichert,R.W. (2007)
α7	R. norvegicus		>>10 μM		ACh (100 μM)	Jimenez,E.C. and Olivera,B.M. and Teichert,R.W. (2007)
α9α10	R. norvegicus		>>10 μM		ACh (100 μM)	Jimenez,E.C. and Olivera,B.M. and Teichert,R.W. (2007)

IC50: Ionotropic glutamate receptors

Target	Organism		IC50		Agonist	Ref
GluN2A	R. norvegicus	(NR1-3b)	>>10 μM		ACh (100 μM)	Jimenez,E.C. and Olivera,B.M. and Teichert,R.W. (2007)
GluN2B	R. norvegicus	(NR1-3b)	>>10 μM		ACh (100 μM)	Jimenez,E.C. and Olivera,B.M. and Teichert,R.W. (2007)

References

Jimenez,E.C., Olivera,B.M. and Teichert,R.W. (2007) AlphaC-conotoxin PrXA: a new family of nicotinic acetylcholine receptor antagonists. Biochemistry 46:8717-8724

El-Aziz,T.M.A., Ravelet,C., Molgo,J., Fiore,E., Pale,S., Amar,M., Al-Khoury,S., Dejeu,J., Fadl,M., Ronjat,M., Taiwe,G.S., Servent,D., Peyrin,E. and De Waard,M (2017) Efficient functional neutralization of lethal peptide toxins in vivo by oligonucleotides Sci Rep 78:7202

Internal links

Protein Precursor	PrXA precursor (4224)
Nucleic acids
Structure

External links

UniProtKB/Swiss-Prot	P0C8S5
Ncbi	P0C8S5

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Tuesday 21 March 2023