RgIA [P6O,del13] (P00030) Protein Card

General Information

Name	RgIA [P6O,del13] (named by ConoServer)
Alternative name(s)	Reg1e,RgIA[Del13]-Amidated
Organism	Conus regius
Organism region	Eastern Pacific
Organism diet	vermivorous
Protein Type	Wild type
Notes	RgIA precursor seems to be the precursor sequence of Reg1e. RgIA has been predicted from the protein prosequence (Ellison et al. 2006). RgIA presents an N-terminal arginine that is usually cleaved by an enzyme (CPE). Reg1e is therefore presumably the real mature peptide resulting from the natural processing of the "RgIA precursor". Inhibited HVA Ca2+ channel current via GABAB receptors in rat DRG neurons with an IC50 of 20.5 nM. (Halai 2011)

Classification

Conopeptide class	conotoxin
Gene superfamily	A superfamily
Cysteine framework	I
Pharmacological family	alpha conotoxin

Sequence

GCCSDORCRYRC(nh2)
Modified residues	position symbol name 6 O 4-Hydroxyproline 13 nh2 C-term amidation
Sequence evidence	protein level
Average Mass	1429.62
Monoisotopic Mass	1428.53
Isoelectric Point	12.20
Extinction Coefficient [280nm]	1490.00

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism	IC50		nhill	Agonist	Ref
α7	R. norvegicus	1.1uM		0.9	200uM Ach	Ellison,M. et al. (2008)
α9α10	R. norvegicus	7.77nM		1.3	50uM Ach	Ellison,M. et al. (2008)

Percentage inhibition: Nicotinic acetylcholine receptors

Target	Organism		% inhibition		Concentration	Ref
α9α10	unidentified	(nAChR was hybrid human α9/rat α10. )	41	+/-15	50 nM	Halai,R. et al. (2011)

References

Franco,A., Pisarewicz,K., Moller,C., Mora,D., Fields,G.B. and Mari,F. (2006) Hyperhydroxylation: a new strategy for neuronal targeting by venomous marine molluscs Prog. Mol. Subcell. Biol. 43:83

Ellison,M., Feng,Z.P., Park,A.J., Zhang,X., Olivera,B.M., McIntosh,J.M. and Norton,R.S. (2008) Alpha-RgIA, a novel conotoxin that blocks the alpha9alpha10 nAChR: structure and identification of key receptor-binding residues. J. Mol. Biol. 377:1216-1227

Halai,R., Callaghan,B., Daly,N.L., Clark,R.J., Adams,D.J. and Craik,D.J. (2011) Effects of cyclization on stability, structure, and activity of α-conotoxin RgIA at the α9α10 nicotinic acetylcholine receptor and GABA(B) receptor. J. Med. Chem. 54:6984-6992

Internal links

Nucleic acids
Structure

External links

UniProtKB/Swiss-Prot	P85011
Ncbi	P85011

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Tuesday 21 March 2023