ImI [D5N] (P00035) Protein Card

General Information
Name ImI [D5N]
Organism synthetic construct
Protein Type Synthetic
Parent ImI

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
GCCSNPRCAWRC(nh2)
Modified residues
positionsymbolname
13nh2C-term amidation
Average Mass 1350.57
Monoisotopic Mass 1349.50
Isoelectric Point 12.50
Extinction Coefficient [280nm] 5500.00

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α7H. sapiens101uM+/-990.3500uM AchRogers,J.P. et al. (2000)

Ki: Nicotinic acetylcholine receptors

TargetOrganismKiCompetitorAgonistRef
α7-5HT3 chimaeraG. gallus31uM+/-7.25nM I-BgtxServent,D. et al. (1998)
H. sapiens262uM+/-103.75nM 125 I-BgtxQuiram,P.A. and Jones,J.J. and Sine,S.M. (1999)

References
Servent,D., Lamthanh,H., Antil,S., Bertrand,D., Corringer,P.J., Changeux,J.P. and Menez,A. (1998) Functional Determinants By Which Snake And Cone Snail Toxins Block The Alpha 7 Neuronal Nicotinic Acetylcholine Receptors J.Physiol. Paris 92:107-111
Johnson,D.S., Martinez,J., Elgoyhen,A.B., Heinemann,S.F. and McIntosh,J.M. (1995) alpha-Conotoxin ImI exhibits subtype-specific nicotinic acetylcholine receptor blockade: preferential inhibition of homomeric alpha 7 and alpha 9 receptors Mol. Pharmacol. 48:194-199
Rogers,J.P., Luginbuhl,P., Pemberton,K., Harty,P., Wemmer,D.E. and Stevens,R.C. (2000) Structure-activity relationships in a peptidic alpha7 nicotinic acetylcholine receptor antagonist J. Mol. Biol. 304:911-926
Quiram,P.A., Jones,J.J. and Sine,S.M. (1999) Pairwise interactions between neuronal alpha7 acetylcholine receptors and alpha-conotoxin ImI. J. Biol. Chem. 274:19517-19524
Quiram,P.A. and Sine,S.M. (1998) Structural elements in alpha-conotoxin ImI essential for binding to neuronal alpha7 receptors. J. Biol. Chem. 273:11007-11011

Internal links
Nucleic acids
Structure NMR SOLUTION STRUCTURE OF ALPHA-CONOTOXIN IM1 POINT MUTATION VARIANT D5N

External links
Ncbi 1E76_A

Tools