GID [(Gla)4A] (P03561) Protein Card

General Information

Name	GID [(Gla)4A] (named by ConoServer)
Alternative name(s)	G1D[Gla4A]
Organism	synthetic construct
Protein Type	Synthetic
Parent	GID

Classification

Conopeptide class	conotoxin
Gene superfamily
Cysteine framework	I
Pharmacological family	alpha conotoxin

Sequence

IRDACCSNPACRVNNOHVC

Modified residues

position	symbol	name
16	O	4-Hydroxyproline

Sequence evidence

protein level

Average Mass

2084.34

Monoisotopic Mass

2082.86

Isoelectric Point

10.94

Extinction Coefficient [280nm]

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism	IC50		n_hill	Agonist	Ref
α3β2	R. norvegicus	0.017uM	+/-0.0041		100nM epibatidine	Banerjee,J. et al. (2014)
		3.0 nM	+/-0.6	1.7	100uM Ach	Millard EL et al. (2009)
α4β2	R. norvegicus	52.0nM	+/-16.5	1	100uM Ach	Millard EL et al. (2009)
		3.5uM	+/-0.9		100nM epibatidine	Banerjee,J. et al. (2014)
α7	R. norvegicus	6.1nM	+/-2.8	1.2	100uM Ach or nicotine	Millard EL et al. (2009)

References

Millard EL, Nevin ST, Loughnan ML, Nicke A, Clark RJ, Alewood PF, Lewis RJ, Adams DJ, Craik DJ, Daly NL (2009) Inhibition of neuronal nicotinic acetylcholine receptor subtypes by alpha-Conotoxin GID and analogues. J Biol Chem. 284:4944-4951

Banerjee,J., Yongye,A.B., Chang,Y.P., Gyanda,R., Medina-Franco,J.L. and Armishaw,C.J. (2014) Design and synthesis of alpha-conotoxin GID analogues as selective alpha4beta2 nicotinic acetylcholine receptor antagonists. Biopolymers 102:78-87

Internal links

Nucleic acids
Structure

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Tuesday 21 March 2023