GID[R12A] (P03565) Protein Card

General Information

Name	GID[R12A]
Alternative name(s)	G1D[R12A]
Organism	synthetic construct
Protein Type	Synthetic
Parent	GID

Classification

Conopeptide class	conotoxin
Gene superfamily
Cysteine framework	I
Pharmacological family	alpha conotoxin

Sequence

IRD(Gla)CCSNPACRVNNOHVC
Modified residues	position symbol name 4 Gla Gamma carboxylic glutamic acid 16 O 4-Hydroxyproline
Average Mass	2186.39
Monoisotopic Mass	2184.85
Isoelectric Point	8.31
Extinction Coefficient [280nm]	NA

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism	IC50		nhill	Agonist	Ref
α3β2	R. norvegicus	10.4nM	+/-0.7	1.2	Ach (100 uM)	Millard EL et al. (2009)
α4β2	R. norvegicus	>1uM				Millard EL et al. (2009)
α7	R. norvegicus	48.8nM	+/-4.2	1.5	Ach (100 uM)	Millard EL et al. (2009)

References

Nicke,A., Loughnan,M.L., Millard,E.L., Alewood,P.F., Adams,D.J., Daly,N.L., Craik,D.J. and Lewis,R.J. (2003) Isolation, Structure, and Activity of GID, a Novel alpha 4/7-Conotoxin with an Extended N-terminal Sequence J. Biol. Chem. 278:3137-3144

Millard EL, Nevin ST, Loughnan ML, Nicke A, Clark RJ, Alewood PF, Lewis RJ, Adams DJ, Craik DJ, Daly NL (2009) Inhibition of neuronal nicotinic acetylcholine receptor subtypes by alpha-Conotoxin GID and analogues. J Biol Chem. 284:4944-4951

Internal links

Nucleic acids
Structure

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Tuesday 28 March 2023