CVIE (P04243) Protein Card

General Information

Name	CVIE (named by ConoServer)
Alternative name(s)	Ca059
Organism	Conus catus
Organism region	Indo-Pacific
Organism diet	piscivorous
Protein Type	Wild type
Protein precursor	CVIE precursor (1188) CVIE precursor (1190) CVIE precursor (1191) CVIE precursor (1194)
Notes	This sequence was published under the name CVIE but another peptide with a different sequence was already published with this name by Bulaj,G. et al. (2001) Delta-conotoxin structure/function through a cladistic analysis Biochemistry 40:13201-13208.

Classification

Conopeptide class	conotoxin
Gene superfamily	O1 superfamily
Cysteine framework	VI/VII
Pharmacological family	omega conotoxin

Sequence

CKGKGASCRRTSYDCCTGSCRSGRC(nh2)

Modified residues

position	symbol	name
26	nh2	C-term amidation

Sequence evidence

protein level

Average Mass

2638.98

Monoisotopic Mass

2637.06

Isoelectric Point

12.54

Extinction Coefficient [280nm]

1490.00

Activity

IC50: Voltage-Gated Calcium Channels

Target	Organism	IC50		n_hill	Ref
Ca_v1.2	R. norvegicus	>3uM			Berecki,G. et al. (2010)
Ca_v1.3	R. norvegicus	>3uM			Berecki,G. et al. (2010)
Ca_v2.2	R. norvegicus	2.6nM	+/-0.5	0.45	Berecki,G. et al. (2010)
		11.1 nM	+/-3.5	0.51	Berecki,G. et al. (2014)
Ca_v2.3	R. norvegicus	>3uM			Berecki,G. et al. (2010)

Percentage inhibition: Voltage-Gated Calcium Channels

Target	Organism	% inhibition		Concentration	Ref
Ca_v2.1	R. norvegicus	10		3uM	Berecki,G. et al. (2010)

References

Berecki,G., Motin,L., Haythornthwaite,A., Vink,S., Bansal,P., Drinkwater,R., Wang,C.I., Moretta,M., Lewis,R.J., Alewood,P.F. and Adams,D.J. (2010) Analgesic ω-conotoxins CVIE and CVIF selectively and voltage-dependently block recombinant and native N-type calcium channels. Mol Pharmacol 77:139-148

Berecki,G., Daly,N.L., Huang,Y.H., Vink,S., Craik,D.J., Alewood,P.F. and Adams,D.J. (2014) Effects of arginine 10 to lysine substitution on ω-conotoxin CVIE and CVIF block of Cav 2.2 channels British journal of pharmacology 171:3313-3327

Himaya, S.W.A., Jin, A.H., Dutertre, S., Giacomotto, J., Mohialdeen, H., Vetter, I., Alewood, P.F. and Lewis, R.J. (2015) Comparative venomics reveals the complex prey capture strategy of the piscivorous cone snail Conus catus. Journal of proteome research 14:4372-4381

Internal links

Protein Precursor	CVIE precursor (1188) CVIE precursor (1190) CVIE precursor (1191) CVIE precursor (1194)
Nucleic acids
Structure

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Wednesday 22 March 2023