MII [H9A,L15A] (P04390) Protein Card

General Information
Name MII [H9A,L15A]
Alternative name(s) [Ala9,Ala15]MII
Organism synthetic construct
Protein Type Synthetic
Parent MII

Classification
Conopeptide class conotoxin
Gene superfamily
Cysteine framework I
Pharmacological family alpha conotoxin

Sequence
GCCSNPVCALEHSNAC(nh2)
Modified residues
positionsymbolname
17nh2C-term amidation
Average Mass 1602.78
Monoisotopic Mass 1601.58
Isoelectric Point 5.63
Extinction Coefficient [280nm] NA

Activity

IC50: Nicotinic acetylcholine receptors

TargetOrganismIC50nhillAgonistRef
α3β2R. norvegicus4850 nM[3540-6630]0.48100uM acetylcholinMcIntosh,J.M. et al. (2004)
α3β4R. norvegicus7.8 uM[5.3-11.5]0.75100uM acetylcholinMcIntosh,J.M. et al. (2004)
α6β4R. norvegicus269 nM[153-476]0.6100uM acetylcholinMcIntosh,J.M. et al. (2004)
α6/α3β2β3R. norvegicus2.40nM[1.68-3.43]0.72100uM acetylcholinMcIntosh,J.M. et al. (2004)

Percentage inhibition: Nicotinic acetylcholine receptors

TargetOrganism% inhibitionConcentrationAgonistRef
α2β2R. norvegicus-3+/-21uM100uM AchMcIntosh,J.M. et al. (2004)
-2+/-210uM100uM AchMcIntosh,J.M. et al. (2004)
α2β4R. norvegicus4+/-21uM100uM AchMcIntosh,J.M. et al. (2004)
3+/-310uM100uM AchMcIntosh,J.M. et al. (2004)
α4β2R. norvegicus-1+/-31uM100uM AchMcIntosh,J.M. et al. (2004)
1+/-410uM100uM AchMcIntosh,J.M. et al. (2004)
α4β4R. norvegicus0+/-21uM100uM AchMcIntosh,J.M. et al. (2004)
1+/-410uM100uM AchMcIntosh,J.M. et al. (2004)
α7R. norvegicus0+/-11uM100uM AchMcIntosh,J.M. et al. (2004)
39+/-310uM100uM AchMcIntosh,J.M. et al. (2004)

References
McIntosh,J.M., Azam,L., Staheli,S., Dowell,C., Lindstrom,J.M., Kuryatov,A., Garrett,J.E., Marks,M.J. and Whiteaker,P. (2004) Analogs of alpha-conotoxin MII are selective for alpha6-containing nicotinic acetylcholine receptors. Mol. Pharmacol. 65:944-952

Internal links
Nucleic acids
Structure

External links

Tools