ImI [P6phi(S)Pro] (P04426) Protein Card

General Information

Name	ImI [P6phi(S)Pro]
Organism	synthetic construct
Protein Type	Synthetic
Parent	ImI

Classification

Conopeptide class	conotoxin
Gene superfamily
Cysteine framework	I
Pharmacological family	alpha conotoxin

Sequence

GCCSD(phi-(S)-Pro)RCAWRC(nh2)
Modified residues	position symbol name 6 phi-(S)-Pro 4-(S)-phenyl-proline 13 nh2 C-term amidation
Average Mass	1427.65
Monoisotopic Mass	1426.51
Isoelectric Point	10.99
Extinction Coefficient [280nm]	5500.00

Activity

IC50: Nicotinic acetylcholine receptors

Target	Organism	IC50		Agonist	Ref
α3β4	R. norvegicus	>300uM		300nM epibatidine	Armishaw,C. et al. (2009)
α7	H. sapiens	>300uM		30uM Ach	Armishaw,C. et al. (2009)

Ki: Nicotinic acetylcholine receptors

Target	Organism	Ki		Competitor	Agonist	Ref
α3β4	R. norvegicus	>100uM		25 pM 3H-epibatidine	5mM S-nicotine	Armishaw,C. et al. (2009)
α7-5HT3 chimaera	R. norvegicus	>100uM		0.5nM 3H-methylcaconitine	5mM S-nicotine	Armishaw,C. et al. (2009)

References

Armishaw,C., Jensen,A.A., Balle,T., Clark,R.J., Harpsøe,K., Skonberg,C., Liljefors,T. and Strømgaard,K. (2009) Rational design of alpha-conotoxin analogues targeting alpha7 nicotinic acetylcholine receptors: improved antagonistic activity by incorporation of proline derivatives. J. Biol. Chem. 284:9498-9512

Internal links

Nucleic acids
Structure

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Thursday 1 June 2023