Conantokin-Rl2 (P05841) Protein Card

General Information

Name	Conantokin-Rl2 (named by ConoServer)
Alternative name(s)	conRl-B,conantokin-Rl-B
Organism	Conus rolani
Protein Type	Wild type
Protein precursor	Conantokin-Rl2 precursor (5849)

Classification

Conopeptide class	conantokin
Gene superfamily	B1 superfamily
Pharmacological family

Sequence

GE(Gla)(Gla)LA(Gla)KAO(Gla)FAR(Gla)LAN(nh2)
Modified residues	position symbol name 3 Gla Gamma carboxylic glutamic acid 4 Gla Gamma carboxylic glutamic acid 7 Gla Gamma carboxylic glutamic acid 10 O 4-Hydroxyproline 11 Gla Gamma carboxylic glutamic acid 15 Gla Gamma carboxylic glutamic acid 19 nh2 C-term amidation
Sequence evidence	nucleic acid level
Average Mass	2238.21
Monoisotopic Mass	2236.92
Isoelectric Point	2.02
Extinction Coefficient [280nm]	NA

Activity

IC50: Ionotropic glutamate receptors

Target	Organism	IC50		Agonist	Ref
GluN2A	R. norvegicus	~10uM		200uM glutamate	Gowd,K.H. et al. (2012)
GluN2B	R. norvegicus	100nM		200uM glutamate	Gowd,K.H. et al. (2012)
GluN2C	R. norvegicus	>10uM		200uM glutamate	Gowd,K.H. et al. (2012)
GluN2D	R. norvegicus	>10uM		200uM glutamate	Gowd,K.H. et al. (2012)

Synthetic variants

Conantokin-Rl2[K8N,A9Q,del10O]	GE(Gla)(Gla)LA(Gla)NQ(Gla)FAR(Gla)LAN(nh2)
Conantokin-Rl2[K8Nle]	GE(Gla)(Gla)LA(Gla)(Nle)AO(Gla)FAR(Gla)LAN(nh2)
Conantokin-Rl2[L5Y]	GE(Gla)(Gla)YA(Gla)KAO(Gla)FAR(Gla)LAN(nh2)
conantokin-Rl2[O10A]	GE(Gla)(Gla)LA(Gla)KAA(Gla)FAR(Gla)LAN(nh2)
conantokin-Rl2[O10P]	GE(Gla)(Gla)LA(Gla)KAP(Gla)FAR(Gla)LAN(nh2)
conantokin-Rl2[delO10]	GE(Gla)(Gla)LA(Gla)KA(Gla)FAR(Gla)LAN(nh2)

References

Gowd,K.H., Han,T.S., Twede,V., Gajewiak,J., Smith,M.D., Watkins,M., Platt,R.J., Toledo,G., White,H.S., Olivera,B.M. and Bulaj,G. (2012) Conantokins derived from the Asprella clade impart conRl-B, an N-methyl d-aspartate receptor antagonist with a unique selectivity profile for NR2B subunits. Biochemistry 51:4685-4692

Yuan,Y., Balsara,R.D., Zajicek,J., Kunda,S. and Castellino,F.J. (2016) Discerning the Role of the Hydroxyproline Residue in the Structure of Conantokin Rl-B and Its Role in GluN2B Subunit-Selective Antagonistic Activity toward N-Methyl-d-Aspartate Receptors. Biochemistry 55:7112-7122

Kunda,S., Yuan,Y., Balsara,R.D., Zajicek,J. and Castellino,F.J. (2015) Hydroxyproline-induced Helical Disruption in Conantokin Rl-B Affects Subunit-selective Antagonistic Activities toward Ion Channels of N-Methyl-d-aspartate Receptors. J. Biol. Chem. 290:18156-18172

Internal links

Protein Precursor	Conantokin-Rl2 precursor (5849)
Nucleic acids
Structure	The solution structure of the Magnesium-bound Conantokin RLB

External links

Tools

Please cite:
Kaas Q, Yu R, Jin AH, Dutertre S and Craik DJ. ConoServer: updated content, knowledge, and discovery tools in the conopeptide database. Nucleic Acids Research (2012) 40(Database issue):D325-30
Kaas Q, Westermann JC, Halai R, Wang CK and Craik DJ. ConoServer, a database for conopeptide sequences and structures. Bioinformatics (2008) 24(3):445-6

ConoServer is managed at the Institute of Molecular Bioscience IMB, Brisbane, Australia.

The database and computational tools found on this website may be used for academic research only, provided that it is referred to ConoServer, the database of conotoxins (http://www.conoserver.org) and the above reference is cited. For any other use please contact David Craik (d.craik@imb.uq.edu.au).

Contacts:
David Craik
Quentin Kaas

Last updated: Wednesday 27 September 2023